Piotr Topolewski, PhD


Doctoral thesis
2023-06-29Sources of the cell-to-cell heterogeneity in interferon gamma and oncostatin M signaling responses 
supervisor -- Prof. Michał Komorowski, PhD, DSc, IPPT PAN
1487
 
Recent publications
1.Topolewski P., Zakrzewska K.E., Walczak J., Nienałtowski K., Müller-Newen G., Singh A., Komorowski M., Phenotypic variability, not noise, accounts for most of the cell-to-cell heterogeneity in IFN-γ and oncostatin M signaling responses, Science Signaling, ISSN: 1945-0877, DOI: 10.1126/scisignal.abd9303, Vol.15, No.721, pp.eabd9303-1-16, 2022
Abstract:

Cellular signaling responses show substantial cell-to-cell heterogeneity, which is often ascribed to the inherent randomness of biochemical reactions, termed molecular noise, wherein high noise implies low signaling fidelity. Alternatively, heterogeneity could arise from differences in molecular content between cells, termed molecular phenotypic variability, which does not necessarily imply imprecise signaling. The contribution of these two processes to signaling heterogeneity is unclear. Here, we fused fibroblasts to produce binuclear syncytia to distinguish noise from phenotypic variability in the analysis of cytokine signaling. We reasoned that the responses of the two nuclei within one syncytium could approximate the signaling outcomes of two cells with the same molecular content, thereby disclosing noise contribution, whereas comparison of different syncytia should reveal contribution of phenotypic variability. We found that ~90% of the variance in the primary response (which was the abundance of phosphorylated, nuclear STAT) to stimulation with the cytokines interferon-γ and oncostatin M resulted from differences in the molecular content of individual cells. Thus, our data reveal that cytokine signaling in the system used here operates in a reproducible, high-fidelity manner.

Affiliations:
Topolewski P.-IPPT PAN
Zakrzewska K.E.-IPPT PAN
Walczak J.-IPPT PAN
Nienałtowski K.-IPPT PAN
Müller-Newen G.-RWTH Aachen University (DE)
Singh A.-University of Delaware (US)
Komorowski M.-IPPT PAN
2.Topolewski P., Komorowski M., Information-theoretic analyses of cellular strategies for achieving high signaling capacity—dynamics, cross-wiring, and heterogeneity of cellular states, Current Opinion in Systems Biology, ISSN: 2452-3100, DOI: 10.1016/j.coisb.2021.06.003, Vol.27, pp.100352-1-9, 2021
Abstract:

An individual eukaryotic cell senses identity and quantity of ligands through molecular receptors and signaling pathways, dynamically activating signaling effectors. A distinct ligand often activates multiple different effectors, and a distinct effector is activated by numerous different ligands, which results in cross-wired signaling. In apparently identical cells, the activity of signaling effectors can vary considerably, raising questions about the accuracy of cellular signaling and the interpretation of heterogeneous responses, as either functional or simply noise. Cell-to-cell variability of signaling outcomes, signaling dynamics, and cross-wiring all give rise to signaling complexity, complicating the analysis of signaling mechanisms. Here, we consider a simple input–output modeling approach of information theory that is suitable to analyze signaling complexity and highlight recent studies that have advanced our understanding of the role different components of signaling complexity play in achieving effective information transfer along cellular signaling pathways.

Keywords:

signaling pathways, hormones, growth factors or cytokines, signaling dynamics, cross-wired signaling, Shannon information, Fisher information

Affiliations:
Topolewski P.-IPPT PAN
Komorowski M.-IPPT PAN

Conference abstracts
1.Topolewski P., Komorowski M., Cell cycle does not contribute to cell-to-cell heterogeneity of interferon responses, Cytokine, ISSN: 1043-4666, DOI: 10.1016/j.cyto.2017.09.011, Vol.100, pp.Mo-P7-11-100-100, 2017
2.Zakrzewska K.E., Jetka T., Nienałtowski K., Szymańska K., Andryka K., Topolewski P., Głów E., Komorowski M., Sensing and remembering IFNs concentrations, Cytokine, ISSN: 1043-4666, DOI: 10.1016/j.cyto.2017.09.011, Vol.100, pp.Mo-P7-12-100-100, 2017