Edyta Głów, MSc


Recent publications
1.Nienałtowski K., Rigby R.E., Walczak J., Zakrzewska K.E., Głów E., Rehwinkel J., Komorowski M., Fractional response analysis reveals logarithmic cytokine responses in cellular populations, Nature Communications, ISSN: 2041-1723, DOI: 10.1038/s41467-021-24449-2, Vol.12, pp.4175-1-10, 2021
Abstract:

Although we can now measure single-cell signaling responses with multivariate, high-throughput techniques our ability to interpret such measurements is still limited. Even interpretation of dose–response based on single-cell data is not straightforward: signaling responses can differ significantly between cells, encompass multiple signaling effectors, and have dynamic character. Here, we use probabilistic modeling and information-theory to introduce fractional response analysis (FRA), which quantifies changes in fractions of cells with given response levels. FRA can be universally performed for heterogeneous, multivariate, and dynamic measurements and, as we demonstrate, quantifies otherwise hidden patterns in single-cell data. In particular, we show that fractional responses to type I interferon in human peripheral blood mononuclear cells are very similar across different cell types, despite significant differences in mean or median responses and degrees of cell-to-cell heterogeneity. Further, we demonstrate that fractional responses to cytokines scale linearly with the log of the cytokine dose, which uncovers that heterogeneous cellular populations are sensitive to fold-changes in the dose, as opposed to additive changes.

Affiliations:
Nienałtowski K.-other affiliation
Rigby R.E.-University of Oxford (GB)
Walczak J.-IPPT PAN
Zakrzewska K.E.-IPPT PAN
Głów E.-IPPT PAN
Rehwinkel J.-University of Oxford (GB)
Komorowski M.-IPPT PAN

Conference abstracts
1.Zakrzewska K.E., Jetka T., Nienałtowski K., Szymańska K., Andryka K., Topolewski P., Głów E., Komorowski M., Sensing and remembering IFNs concentrations, Cytokine, ISSN: 1043-4666, DOI: 10.1016/j.cyto.2017.09.011, Vol.100, pp.Mo-P7-12-100-100, 2017
2.Andryka K., Głów E., Nienałtowski K., Jetka T., Komorowski M., Sensing accuracy of interferons' concentrations, Cytokine, ISSN: 1043-4666, DOI: 10.1016/j.cyto.2015.08.238, Vol.76, pp.108, 2015
Abstract:

Interferons exhibit their key role of immune modulators through activation of the Jak-Stat signalling pathway. We know substantial amount of molecular details regarding functioning of the pathway. However, to what extend the action of the pathway is dose dependent at the single cell level remains largely unclear. Specifically it is not know if single cells respond in a digital fashion or their output is continuously dependent on the stimulant’s concentration. We have combined an information-theoretic framework with high-throughput confocal imaging of mouse embryonic fibroblasts to provide a thorough, single-cell analysis of the Jak-Stat signalling in response to interferon beta and interferon gamma. We showed that in a baseline state single cells have information hardly sufficient to distinguish between presence or absence of interferons. However they can be put in an alert state by an action of interferons, which allows them to respond more in an analogous fashion. Our results show that the accuracy with which signalling pathways transmit information is not fixed but can be modulated on the contextual basis.

Affiliations:
Andryka K.-other affiliation
Głów E.-IPPT PAN
Nienałtowski K.-IPPT PAN
Jetka T.-other affiliation
Komorowski M.-IPPT PAN
3.Głów E., Jetka T., Komorowski M., Sensitisation in the IFN-alpha/b, IFN-gamma crosstalk reveals mechanisms for enhanced information processing capacity of the STAT1, STAT2 signalling pathway, FEBS Journal, ISSN: 1742-464X, Vol.281, No.Supplement: 1, Special Issue: SI, pp.640, 2014
Keywords:

Interferon, Jak/Stat, Stimulation

Affiliations:
Głów E.-other affiliation
Jetka T.-other affiliation
Komorowski M.-IPPT PAN