Partner: Sandra Stamnitz

Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences (PL)

Recent publications
1.Bar Julia K.K., Lis-Nawara A., Kowalczyk T., Grelewski Piotr G.G., Stamnitz S., Gerber H., Klimczak A., Osteogenic Potential of Human Dental Pulp Stem Cells (hDPSCs) Growing on Poly L-Lactide-Co-Caprolactone and Hyaluronic Acid (HYAFF-11TM) Scaffolds, International Journal of Molecular Sciences, ISSN: 1422-0067, DOI: 10.3390/ijms242316747, Vol.24, No.23, pp.16747-1-20, 2023
Abstract:

Bone tissue engineering using different scaffolds is a new therapeutic approach in regenerative medicine. This study explored the osteogenic potential of human dental pulp stem cells (hDPSCs) grown on a hydrolytically modified poly(L-lactide-co-caprolactone) (PLCL) electrospun scaffold and a non-woven hyaluronic acid (HYAFF-11™) mesh. The adhesion, immunophenotype, and osteogenic differentiation of hDPSCs seeded on PLCL and HYAFF-11™ scaffolds were analyzed. The results showed that PLCL and HYAFF-11™ scaffolds significantly supported hDPSCs adhesion; however, hDPSCs’ adhesion rate was significantly higher on PLCL than on HYAFF-11™. SEM analysis confirmed good adhesion of hDPSCs on both scaffolds before and after osteogenesis. Alizarin red S staining showed mineral deposits on both scaffolds after hDPSCs osteogenesis. The mRNA levels of runt-related transcription factor 2 (Runx2), collagen type I (Coll-I), osterix (Osx), osteocalcin (Ocn), osteopontin (Opn), bone sialoprotein (Bsp), and dentin sialophosphoprotein (Dspp) gene expression and their proteins were higher in hDPSCs after osteogenic differentiation on both scaffolds compared to undifferentiated hDPSCs on PLCL and HYAFF-11™. These results showed that PLCL scaffolds provide a better environment that supports hDPSCs attachment and osteogenic differentiation than HYAFF-11™. The high mRNA of early osteogenic gene expression and mineral deposits observed after hDPSCs osteogenesis on a PLCL mat indicated its better impact on hDPSCs’ osteogenic potential than that of HYAFF-11™, and hDPSC/PLCL constructs might be considered in the future as an innovative approach to bone defect repair.

Keywords:

dental stem cells, hDPSCs, osteogenesis, PLCL scaffold, HYAFF-11 scaffold

Affiliations:
Bar Julia K.K.- ()
Lis-Nawara A.- ()
Kowalczyk T.-IPPT PAN
Grelewski Piotr G.G.- ()
Stamnitz S.-Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences (PL)
Gerber H.-other affiliation
Klimczak A.-Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences (PL)
2.Bar J.K., Kowalczyk T., Grelewski P.G., Stamnitz S., Paprocka M., Lis J., Lis-Nawara A., An S., Klimczak A., Characterization of biological properties of dental pulp stem cells grown on an electrospun poly(l-lactide-co-caprolactone) scaffold, Materials, ISSN: 1996-1944, DOI: 10.3390/ma15051900, Vol.15, No.5, pp.1900-1-28, 2022
Abstract:

Poly(l-lactide-co-caprolactone) (PLCL) electrospun scaffolds with seeded stem cells have drawn great interest in tissue engineering. This study investigated the biological behavior of human dental pulp stem cells (hDPSCs) grown on a hydrolytically-modified PLCL nanofiber scaffold. The hDPSCs were seeded on PLCL, and their biological features such as viability, proliferation, adhesion, population doubling time, the immunophenotype of hDPSCs and osteogenic differentiation capacity were evaluated on scaffolds. The results showed that the PLCL scaffold significantly supported hDPSC viability/proliferation. The hDPSCs adhesion rate and spreading onto PLCL increased with time of culture. hDPSCs were able to migrate inside the PLCL electrospun scaffold after 7 days of seeding. No differences in morphology and immunophenotype of hDPSCs grown on PLCL and in flasks were observed. The mRNA levels of bone-related genes and their proteins were significantly higher in hDPSCs after osteogenic differentiation on PLCL compared with undifferentiated hDPSCs on PLCL. These results showed that the mechanical properties of a modified PLCL mat provide an appropriate environment that supports hDPSCs attachment, proliferation, migration and their osteogenic differentiation on the PLCL scaffold. The good PLCL biocompatibility with dental pulp stem cells indicates that this mat may be applied in designing a bioactive hDPSCs/PLCL construct for bone tissue engineering.

Keywords:

hDPSCs, poly(l-lactide-co-caprolactone), electrospun scaffold, biocompatibility, adhesion, proliferation, osteogenic differentiation, tissue engineering

Affiliations:
Bar J.K.- ()
Kowalczyk T.-IPPT PAN
Grelewski P.G.- ()
Stamnitz S.-Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences (PL)
Paprocka M.-Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences (PL)
Lis J.-other affiliation
Lis-Nawara A.- ()
An S.-Sungkyunkwan University (KR)
Klimczak A.-Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences (PL)