Partner: Hanna Gerber


Recent publications
1.Bar Julia K.K., Lis-Nawara A., Kowalczyk T., Grelewski Piotr G.G., Stamnitz S., Gerber H., Klimczak A., Osteogenic Potential of Human Dental Pulp Stem Cells (hDPSCs) Growing on Poly L-Lactide-Co-Caprolactone and Hyaluronic Acid (HYAFF-11TM) Scaffolds, International Journal of Molecular Sciences, ISSN: 1422-0067, DOI: 10.3390/ijms242316747, Vol.24, No.23, pp.16747-1-20, 2023
Abstract:

Bone tissue engineering using different scaffolds is a new therapeutic approach in regenerative medicine. This study explored the osteogenic potential of human dental pulp stem cells (hDPSCs) grown on a hydrolytically modified poly(L-lactide-co-caprolactone) (PLCL) electrospun scaffold and a non-woven hyaluronic acid (HYAFF-11™) mesh. The adhesion, immunophenotype, and osteogenic differentiation of hDPSCs seeded on PLCL and HYAFF-11™ scaffolds were analyzed. The results showed that PLCL and HYAFF-11™ scaffolds significantly supported hDPSCs adhesion; however, hDPSCs’ adhesion rate was significantly higher on PLCL than on HYAFF-11™. SEM analysis confirmed good adhesion of hDPSCs on both scaffolds before and after osteogenesis. Alizarin red S staining showed mineral deposits on both scaffolds after hDPSCs osteogenesis. The mRNA levels of runt-related transcription factor 2 (Runx2), collagen type I (Coll-I), osterix (Osx), osteocalcin (Ocn), osteopontin (Opn), bone sialoprotein (Bsp), and dentin sialophosphoprotein (Dspp) gene expression and their proteins were higher in hDPSCs after osteogenic differentiation on both scaffolds compared to undifferentiated hDPSCs on PLCL and HYAFF-11™. These results showed that PLCL scaffolds provide a better environment that supports hDPSCs attachment and osteogenic differentiation than HYAFF-11™. The high mRNA of early osteogenic gene expression and mineral deposits observed after hDPSCs osteogenesis on a PLCL mat indicated its better impact on hDPSCs’ osteogenic potential than that of HYAFF-11™, and hDPSC/PLCL constructs might be considered in the future as an innovative approach to bone defect repair.

Keywords:

dental stem cells, hDPSCs, osteogenesis, PLCL scaffold, HYAFF-11 scaffold

Affiliations:
Bar Julia K.K.- ()
Lis-Nawara A.- ()
Kowalczyk T.-IPPT PAN
Grelewski Piotr G.G.- ()
Stamnitz S.-Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences (PL)
Gerber H.-other affiliation
Klimczak A.-Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences (PL)