Partner: Aleksandra Kruk

Warsaw University of Technology (PL)

Recent publications
1.Wasyłeczko M., Krysiak Z.J., Łukowska E., Gruba M., Sikorska W., Kruk A., Dulnik J., Czubak J., Chwojnowski A., Three-dimensional scaffolds for bioengineering of cartilage tissue, Biocybernetics and Biomedical Engineering, ISSN: 0208-5216, DOI: 10.1016/j.bbe.2022.03.004, Vol.42, No.2, pp.494-511, 2022
Abstract:

The cartilage tissue is neither supplied with blood nor innervated, so it cannot heal by itself. Thus, its reconstruction is highly challenging and requires external support. Cartilage diseases are becoming more common due to the aging population and obesity. Among young people, it is usually a post-traumatic complication. Slight cartilage damage leads to the spontaneous formation of fibrous tissue, not resistant to abrasion and stress, resulting in cartilage degradation and the progression of the disease. For these reasons, cartilage regeneration requires further research, including use of new type of biomaterials for scaffolds. This paper shows cartilage characteristics within its most frequent problems and treatment strategies, including a promising method that combines scaffolds and human cells. Structure and material requirements, manufacturing methods, and commercially available scaffolds were described. Also, the comparison of poly(L-lactide) (PLLA) and polyethersulfone (PES) 3D membranes obtained by a phase inversion method using nonwovens as a pore-forming additives were reported. The scaffolds' structure and the growth ability of human chondrocytes were compared. Scaffolds' structure, cells morphology, and protein presence in the membranes were examined with a scanning electron microscope. The metabolic activity of cells was tested with the MTT assay. The structure of the scaffolds and the growth capacity of human chondrocytes were compared. Obtained results showed higher cell activity and protein content for PES scaffolds than for PLLA. The PES membrane had better mechanical properties (e.g. ripping), greater chondrocytes proliferation, and thus a better secretion of proteins which build up the cartilage structure.

Keywords:

3D-scaffolds, membrane structure, polyethersulfone, poly(L-lactide), chondrocyte culture, cartilage regeneration

Affiliations:
Wasyłeczko M.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Krysiak Z.J.-other affiliation
Łukowska E.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Gruba M.-Gruca Orthopedic and Trauma Teaching Hospital, Centre of Postgraduate Medical Education (PL)
Sikorska W.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Kruk A.-Warsaw University of Technology (PL)
Dulnik J.-IPPT PAN
Czubak J.-Gruca Orthopedic and Trauma Teaching Hospital, Centre of Postgraduate Medical Education (PL)
Chwojnowski A.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
2.Gadomska‐Gajadhur A., Kruk A., Ruśkowski P., Sajkiewicz P., Dulnik J., Chwojnowski A., Original method of imprinting pores in scaffolds for tissue engineering, Polymers for Advanced Technologies, ISSN: 1042-7147, DOI: 10.1002/pat.5091, pp.1-13, 2020
Abstract:

Results of the preparation of biodegradable porous scaffolds using an original modification of a wet phase inversion method were presented. Influence of gelatin non‐woven as a non‐classic pore precursor and polyvinylpyrrolidone, Pluronic as classic pore precursors on the structure of obtained scaffolds was analyzed. It was shown that the addition of gelatin non‐wovens enables the preparation of scaffolds, which allow for the growth of cells (size, distribution, and shape of pores). Mechanical properties of the obtained cell scaffolds were determined. The influence of pore precursors on mass absorption of scaffolds against isopropanol and plasma was investigated. Interaction of scaffolds with a T‐lymphocyte line (Jurkat) and with fibroblasts (L929) was investigated. Obtained scaffolds are not cytotoxic and can be used as implants, for example, the regeneration of cartilage tissue.

Keywords:

cell cultures, cytotoxic, fibroblasts, imprinted scaffolds

Affiliations:
Gadomska‐Gajadhur A.-other affiliation
Kruk A.-Warsaw University of Technology (PL)
Ruśkowski P.-Warsaw University of Technology (PL)
Sajkiewicz P.-IPPT PAN
Dulnik J.-IPPT PAN
Chwojnowski A.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
3.Kruk A., Gadomska-Gajadhur A., Dulnik J., Ruśkowski P., The influence of the molecular weight of polymer on the morphology, functional properties and L929 fibroblasts growth on polylactide membranes for tissue engineering, International Journal of Polymeric Materials and Polymeric Biomaterials, ISSN: 0091-4037, DOI: 10.1080/00914037.2020.1798440, pp.1-13, 2020
Abstract:

The main goal of tissue engineering (TE) is supporting the regeneration of damaged tissues that are difficult to regenerate. The experimental results of the preparation of semi-permeable membranes for cell cultures are presented. The effect of the PLA molecular weight and addition of pore precursors on the morphology of the membranes was studied. The pore precursor of choice was polyvinylpyrrolidone (PVP). It was found that semi-permeable membranes for application in tissue engineering can be prepared with polylactides of molecular weight more significant than 37,000 g/mol. Moreover, it was observed that the growth of the molecular weight of the polymer, the porosity, the size of the pores, the Young modulus and maximum tensile increased. Additionally, to obtain a better morphology of the membranes, PVP should be added to the polymeric solution. Positive growth of L929 fibroblast cells on the obtained scaffolds was shown.

Keywords:

biodegradable polymers, cell cultures, L929 fibroblasts, polylactide, scaffolds, tissue engineering

Affiliations:
Kruk A.-Warsaw University of Technology (PL)
Gadomska-Gajadhur A.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Dulnik J.-IPPT PAN
Ruśkowski P.-Warsaw University of Technology (PL)
4.Gadomska‐Gajadhur A., Kruk A., Dulnik J., Chwojnowski A., New polyester biodegradable scaffolds for chondrocyte culturing: preparation, properties, and biological activity, JOURNAL OF APPLIED POLYMER SCIENCE, ISSN: 0021-8995, DOI: 10.1002/app.50089, pp.e50089-1-14, 2020
Abstract:

An innovative modification of the wet inversion phase method, consisting in the use of a polymer nano‐nonwoven as a nonclassic pore precursor. Mechanical properties of the obtained scaffolds were determined, their hydrophilic properties (serum absorbability) were tested, and the content of residues of materials used in the scaffold preparation was determined. Nontoxicity of the developed scaffolds toward T lymphocyte cells was proved. Cultures of primary chondrocytes were obtained successfully. It was proved that an addition of a polymer nano‐nonwoven changes the properties of the scaffolds favorably in respect of their subsequent application in tissue engineering.

Keywords:

cartilage regeneration, chondrocytes, nano-nonwoven, polyvinylpyrrolidone, T lymphocytes

Affiliations:
Gadomska‐Gajadhur A.-other affiliation
Kruk A.-Warsaw University of Technology (PL)
Dulnik J.-IPPT PAN
Chwojnowski A.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
5.Kruk A., Gadomska-Gajadhur A., Rykaczewska I., Dulnik J., Ruśkowski P., Synoradzki L., Influence of liquid pore precursors on morphology and mechanical properties of 3D scaffolds obtained by dry inversion phase method, Journal of Biomedical Materials Research Part B: Applied Biomaterials, ISSN: 1552-4973, DOI: 10.1002/jbm.b.34200, Vol.107, No.4, pp.1079-1087, 2019
Abstract:

Polyester 3D scaffolds were obtained by dry inversion phase method. The influence of a polymer and liquid pore precursor type on the 3D scaffolds morphology, porosity and mechanical properties was tested. Polymers and precursors forming a porous structure were identified. It was found that 3D scaffolds having the most preferable structure for cell cultures were obtained from polylactide with the addition of n‐butanol as the liquid pore precursor.

Keywords:

liquid pore precursors, mechanical properties, dry inversion phase method, 3D scaffolds

Affiliations:
Kruk A.-Warsaw University of Technology (PL)
Gadomska-Gajadhur A.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Rykaczewska I.-Warsaw University of Technology (PL)
Dulnik J.-IPPT PAN
Ruśkowski P.-Warsaw University of Technology (PL)
Synoradzki L.-Warsaw University of Technology (PL)
6.Kruk A., Gadomska-Gajadhur A., Dulnik J., Rykaczewska I., Ruśkowski P., Sebai A., Synoradzki L., Ocena właściwości użytkowych rusztowań komórkowych o strukturze gąbczastej oraz wzrostu na nich fibroblastów, POLIMERY, ISSN: 0032-2725, DOI: 10.14314/polimery.2018.4.3, Vol.63, No.4, pp.18-22, 2018
Abstract:

Zbadano wpływ dodatku ciekłych prekursorów porów na morfologię, porowatość i właściwości mechaniczne polilaktydowych rusztowań komórkowych. Rusztowania otrzymano metodą mokrej inwersji faz w wariancie freeze extraction. Oceniono cytotoksyczność wybranych rusztowań w stosunku do fibroblastów mysich oraz ich przydatność do hodowli komórkowych. Wykazano, że dodatek prekursora porów dopolilaktydu korzystnie zmienia morfologię wytworzonych rusztowań, jednocześnie pogarszając ich wytrzymałość mechaniczną. Stwierdzono, że polilaktydowe rusztowania komórkowe z powodzeniem mogą być wykorzystywane do hodowli komórkowych.

Keywords:

usztowania komórkowe, polilaktyd, hodowle komórkowe, fibroblasty

Affiliations:
Kruk A.-Warsaw University of Technology (PL)
Gadomska-Gajadhur A.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Dulnik J.-IPPT PAN
Rykaczewska I.-Warsaw University of Technology (PL)
Ruśkowski P.-Warsaw University of Technology (PL)
Sebai A.-Wroclaw University of Science and Technology (PL)
Synoradzki L.-Warsaw University of Technology (PL)

Conference papers
1.Chwojnowski A., Kruk A., Wojciechowski C., Łukowska E., Dulnik J., Sajkiewicz P., The dependence of the membrane structure on the non-woven forming the macropores in the 3D scaffolds preparation, Desalination and Water Treatment, ISSN: 1944-3994, DOI: 10.5004/dwt.2017.11394, Vol.64, pp.324-331, 2017
Abstract:

Three types of membrane structures with wide pores were compared in this study. One of the membranes was obtained from polyethersulfone using cellulose fibers as the macropore precursors. Two of the fibers were obtained from poly(L-lactide). As the macropore precursors olyvinylpyrrolidone (1.2 MDa) and pork gelatin non-woven were used, the influence of non-woven fibers on the structure of membranes was shown. Necessity of specific membrane structure application was explained. The hoice of polymers and co-polymers with a range of biodegradation times can determine the scaffold type suitable for the age of a patient.

Keywords:

Polysulfone membrane, Polyester membranes, Membrane structures, Biodegradable membranes, 3D scaffold

Affiliations:
Chwojnowski A.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Kruk A.-Warsaw University of Technology (PL)
Wojciechowski C.-other affiliation
Łukowska E.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Dulnik J.-IPPT PAN
Sajkiewicz P.-IPPT PAN
2.Kruk A., Gadomska-Gajadhur A., Ruśkowski P., Chwojnowski A., Dulnik J., Synoradzki L., Preparation of biodegradable semi-permeable membranes as 3D scaffolds for cell cultures, Desalination and Water Treatment, ISSN: 1944-3994, DOI: 10.5004/dwt.2017.11415, Vol.64, pp.317-323, 2017
Abstract:

Results of the preparation of semi-permeable membranes made of biodegradable polymers membranes were presented. Among known polyesters, polylactide was selected for research. The membranes were obtained using wet phase inversion method. The influence of polyvinylpyrrolidone and polymeric nano-non-wovens as pores precursors on the structure of obtained membranes was analysed. It was shown, that utilisation of polymeric nano-non-wovens enabled preparation of semi-permeable membranes, which could be used as wide-pore 3D-type cellular scaffolds.

Keywords:

Biodegradable polymers membranes, Biodegradable polyesters, Porous three-dimensional scaffolds, Inversion phase method

Affiliations:
Kruk A.-Warsaw University of Technology (PL)
Gadomska-Gajadhur A.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Ruśkowski P.-Warsaw University of Technology (PL)
Chwojnowski A.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Dulnik J.-IPPT PAN
Synoradzki L.-Warsaw University of Technology (PL)

Conference abstracts
1.Chwojnowski A., Kruk A., Wojciechowski C., Łukowska E., Dulnik J., Sajkiewicz P., The dependence of the membrane structure on the non-woven forming the macropores in the 3D scaffolds preparation, MEMPEP 2016, XI-th Conference on Membranes and Membrane Processes in Environmental Protection, 2016-06-15/06-18, Zakopane (PL), pp.23, 2016
2.Kruk A., Gadomska-Gajadhur A., Ruśkowski P., Chwojnowski A., Dulnik J., Synoradzki L., Preparation of biodegradable semi-permeable membranes as 3D scaffolds for cell cultures, MEMPEP 2016, XI-th Conference on Membranes and Membrane Processes in Environmental Protection, 2016-06-15/06-18, Zakopane (PL), pp.24, 2016

Patents
Filing No./Date
Filing Publication
Autor(s)
Title
Protection Area, Applicant Name
Patent Number
Date of Grant
pdf
414353
2015-10-13
BUP 09/2017
2017-04-24
Chwojnowski A., Łukowska E., Wojciechowski C., Gadomska-Gajadhur A., Kruk A., Ruśkowski P., Synoradzki L., Denis P., Dulnik J., Sajkiewicz P.
Sposób otrzymywania szerokoporowatego, poliestrowego rusztowania komórkowego
PL, Instytut Biocybernetyki i Inżynierii Biomedycznej im. Macieja Nałęcza PAN, Politechnika Warszawska, Instytut Podstawowych Problemów Techniki PAN
228884
WUP 05/2018
2018-05-30