Partner: Andrea Barbetta

Sapienza University of Rome (IT)

Ostatnie publikacje
1.Cidonio G., Costantini M., Pierini F., Scognamiglio C., Agarwal T., Barbetta A., 3D printing of biphasic inks: beyond single-scale architectural control, Journal of Materials Chemistry C, ISSN: 2050-7526, DOI: 10.1039/d1tc02117f, Vol.9, No.37, pp.12489-12508, 2021

Streszczenie:

To date, Additive Manufacturing (AM) has come to the fore as a major disruptive technology embodying two main research lines – developing increasingly sophisticated printing technologies and new processable materials. The latter has fostered a tremendous leap in AM technological advancement, allowing 3D printing to play a central role in dictating the tailorable settings for material design. In particular, the manufacturing of three-dimensional (3D) objects with functional hierarchical porous structure is of the utmost importance for numerous research areas, including tissue engineering, catalysis, aerospace, environmental science, electrochemistry, energy and sound absorption and light engineering materials. Biphasic inks such as emulsions, foams, and solid dispersions represent viable templating systems to realise multiscale porosity. The combination of AM techniques and biphasic inks provide pivotal control over multiple levels of material structure and function, enabling the use of advanced materials with unprecedented 3D architectures as well as physical, chemical, and mechanical properties. The related potential benefits are significant, with functional perspectives for a wide variety of research fields. In this concise review, we provide an updated overview of the employment of biphase inks and show how they are adapted to different AM technologies or vice versa.

Afiliacje autorów:

Cidonio G.-other affiliation
Costantini M.-Sapienza University of Rome (IT)
Pierini F.-IPPT PAN
Scognamiglio C.-other affiliation
Agarwal T.-other affiliation
Barbetta A.-Sapienza University of Rome (IT)
140p.
2.Kosik-Kozioł A., Costantini M., Mróz A., Idaszek J., Heljak M., Jaroszewicz J., Kijeńska E., Szöke K., Frerker N., Barbetta A., Brinchmann J.E., Święszkowski W., 3D bioprinted hydrogel model incorporating β-tricalcium phosphate for calcified cartilage tissue engineering, Biofabrication, ISSN: 1758-5082, DOI: 10.1088/1758-5090/ab15cb, Vol.11, No.3, pp.035016-1-29, 2019

Streszczenie:

One promising strategy to reconstruct osteochondral defects relies on 3D bioprinted three-zonal structures comprised of hyaline cartilage, calcified cartilage, and subchondral bone. So far, several studies have pursued the regeneration of either hyaline cartilage or bone in vitro while—despite its key role in the osteochondral region—only few of them have targeted the calcified layer. In this work, we present a 3D biomimetic hydrogel scaffold containing β-tricalcium phosphate (TCP) for engineering calcified cartilage through a co-axial needle system implemented in extrusion-based bioprinting process. After a thorough bioink optimization, we showed that 0.5% w/v TCP is the optimal concentration forming stable scaffolds with high shape fidelity and endowed with biological properties relevant for the development of calcified cartilage. In particular, we investigate the effect induced by ceramic nano-particles over the differentiation capacity of bioprinted bone marrow-derived human mesenchymal stem cells in hydrogel scaffolds cultured up to 21 d in chondrogenic media. To confirm the potential of the presented approach to generate a functional in vitro model of calcified cartilage tissue, we evaluated quantitatively gene expression of relevant chondrogenic (COL1, COL2, COL10A1, ACAN) and osteogenic (ALPL, BGLAP) gene markers by means of RT-qPCR and qualitatively by means of fluorescence immunocytochemistry.

Słowa kluczowe:

alginate, gelatin methacrylate, ß-tricalcium phosphate TCP, bioprinting, coaxial needle, calcified cartilage

Afiliacje autorów:

Kosik-Kozioł A.-other affiliation
Costantini M.-Sapienza University of Rome (IT)
Mróz A.-other affiliation
Idaszek J.-other affiliation
Heljak M.-Politechnika Warszawska (PL)
Jaroszewicz J.-other affiliation
Kijeńska E.-other affiliation
Szöke K.-other affiliation
Frerker N.-other affiliation
Barbetta A.-Sapienza University of Rome (IT)
Brinchmann J.E.-other affiliation
Święszkowski W.-other affiliation
140p.
3.Costantini M., Guzowski J., Żuk P.J., Mozetic P., De Panfilis S., Jaroszewicz J., Heljak M., Massimi M., Pierron M., Trombetta M., Dentini M., Święszkowski W., Rainer A., Garstecki P., Barbetta A., Electric Field Assisted Microfluidic Platform for Generation of Tailorable Porous Microbeads as Cell Carriers for Tissue Engineering, Advanced Functional Materials, ISSN: 1616-301X, DOI: 10.1002/adfm.201800874, Vol.28, pp.1800874-1-13, 2018

Streszczenie:

Injection of cell‐laden scaffolds in the form of mesoscopic particles directly to the site of treatment is one of the most promising approaches to tissue regeneration. Here, a novel and highly efficient method is presented for preparation of porous microbeads of tailorable dimensions (in the range ≈300–1500 mm) and with a uniform and fully interconnected internal porous texture. The method starts with generation of a monodisperse oil‐in‐water emulsion inside a flow‐focusing microfluidic device. This emulsion is later broken‐up, with the use of electric field, into mesoscopic double droplets, that in turn serve as a template for the porous microbeads. By tuning the amplitude and frequency of the electric pulses, the template droplets and the resulting porous bead scaffolds are precisely produced. Furthermore, a model of pulsed electrodripping is proposed that predicts the size of the template droplets as a function of the applied voltage. To prove the potential of the porous microbeads as cell carries, they are tested with human mesenchymal stem cells and hepatic cells, with their viability and degree of microbead colonization being monitored. Finally, the presented porous microbeads are benchmarked against conventional microparticles with nonhomogenous internal texture, revealing their superior performance.

Afiliacje autorów:

Costantini M.-Sapienza University of Rome (IT)
Guzowski J.-Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Żuk P.J.-IPPT PAN
Mozetic P.-Università Campus Bio-Medico di Roma (IT)
De Panfilis S.-Sapienza Istituto Italiano di Tecnologia (IT)
Jaroszewicz J.-other affiliation
Heljak M.-Politechnika Warszawska (PL)
Massimi M.-University of L’Aquila (IT)
Pierron M.-Telecom Physique Strasbourg (FR)
Trombetta M.-Università Campus Bio-Medico di Roma (IT)
Dentini M.-Sapienza University of Rome (IT)
Święszkowski W.-other affiliation
Rainer A.-Università Campus Bio-Medico di Roma (IT)
Garstecki P.-Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Barbetta A.-Sapienza University of Rome (IT)
45p.
4.Kosik-Kozioł A., Costantini M., Bolek T., Szöke K., Barbetta A., Brinchmann J., Święszkowski W., PLA short sub-micron fiber reinforcement of 3D bioprinted alginate constructs for cartilage regeneration, Biofabrication, ISSN: 1758-5082, DOI: 10.1088/1758-5090/aa90d7, Vol.9, No.4, pp.044105-1-13, 2017

Streszczenie:

In this study, we present an innovative strategy to reinforce 3D-printed hydrogel constructs for cartilage tissue engineering by formulating composite bioinks containing alginate and short sub-micron polylactide (PLA) fibers. We demonstrate that Young's modulus obtained for pristine alginate constructs (6.9 ± 1.7 kPa) can be increased threefold (up to 25.1 ± 3.8 kPa) with the addition of PLA short fibers. Furthermore, to assess the performance of such materials in cartilage tissue engineering, we loaded the bioinks with human chondrocytes and cultured in vitro the bioprinted constructs for up to 14 days. Live/dead assays at day 0, 3, 7 and 14 of in vitro culture showed that human chondrocytes were retained and highly viable (∼80%) within the 3D deposited hydrogel filaments, thus confirming that the fabricated composites materials represent a valid solution for tissue engineering applications. Finally, we show that the embedded chondrocytes during all the in vitro culture maintain a round morphology, a key parameter for a proper deposition of neocartilage extracellular matrix.

Słowa kluczowe:

alginate, PLA, short fibers, hydrogel reinforcement, chondrocytes

Afiliacje autorów:

Kosik-Kozioł A.-other affiliation
Costantini M.-Sapienza University of Rome (IT)
Bolek T.-other affiliation
Szöke K.-other affiliation
Barbetta A.-Sapienza University of Rome (IT)
Brinchmann J.-other affiliation
Święszkowski W.-other affiliation

Lista rozdziałów w ostatnich monografiach
1.
625
Costantini M., Testa S., Rinoldi C., Celikkin N., Idaszek J., Colosi C., Gargioli C., Święszkowski W., Barbetta A., Biomaterials Science Series, Biofabrication and 3D Tissue Modeling, rozdział: 3D Tissue Modelling of Skeletal Muscle Tissue, Royal Society of Chemistry, Edited by Dong-Woo Cho, 3, pp.184-215, 2019