Institute of Fundamental Technological Research

Recent biomedical hydrogels applications require the development of nanostructures with controlled diameter and adjustable mechanical properties. Here we present a technique for the production of flexible nanofilaments to be used as drug carriers or in microfluidics, with deformability and elasticity resembling those of long DNA chains. The fabrication method is based on the core-shell electrospinning technique with core solution polymerisation post electrospinning. Produced from the nanofibers highly deformable hydrogel nanofilaments are characterised by their Brownian motion and bending dynamics. The evaluated mechanical properties are compared with AFM nanoindentation tests.

Correction: Hydrogel Nanofilaments via Core-Shell Electrospinning, Nakielski P., Pawłowska S., Pierini F., Liwińska W., Hejduk P., Zembrzycki K., Zabost E., Kowalewski T.A., PLOS ONE, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0133458, Vol.10, No.7, pp.e0133458-1-2, 2015

Gels, Nanomaterials, Atomic force microscopy, Polymerization, Bending, Mass diffusivity, Mechanical properties, Hydrodynamics

We used the principles of electrospinning to produce materials for applications in regenerative medicine of urinary bladder wall, ureter, wound dressing and potential applications in cancer therapy. Our research is based on biodegradable polymers produced by ring-opening polymerization. Scaffolds of poly(L-lactide-co-caprolactone) (PLCL) gradually degrade leaving no artificial material behind to be replaced by natural extracellular collagen matrix. We formed flat membranes of micro- and nanofibers to carry out regeneration of urinary bladder wall as animal model of cancer treatment. Grafts were tested for biocompatibility and aimed for guided cell growth, yet we were unsuccessful in mechanical compliance of nanomaterial and reconstructed tissue. We tested tubular scaffolds made of nanofibers aimed for ureter tissue engineering. We found stem cells seeding unnecessary. The results of nanomaterial implantation on animal model were better than for collagen matrices. Animal model was also tested for use of nanofibers of human serum albumin as wound dressing. The native structure of the protein was retained to maintain its anti-adhesive properties, despite poor mechanical characteristics. Nanomaterial caused no inflammation and was resorbed during 16 days. Last application of presented materials was targeted drug delivery system made of PLCL nanofibers. Release of anticancer drug complexed with nanoparticles is to be triggered by tumor cells. Such nanomaterial is potential drug delivery system. Acknowledgements: The authors wishes to thank for the cooperation: T. Chmielewski, P. Nakielski, K. Zembrzycki, G. Mikulowski and prof. T. A. Kowalewski from IPPT PAN. The project was partially supported by the National Centre for Research and Development. Grant No. STRATEGMED1/235368/8/NCBR/2014.

electrospinning, nanofibers, regenerative medicine, wound dressing, urology, biodegradable polymers, animal model.