Partner: W. Leśniak


Recent publications
1.Żabka M., Leśniak W., Prus W., Kuźnicki J., Filipek A., Sgt1 has co-chaperone properties and is up-regulated by heat shock, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ISSN: 0006-291X, DOI: 10.1016/j.bbrc.2008.03.055, Vol.370, pp.179-183, 2008
Abstract:

The Sgt1 protein is a binding partner of heat shock proteins such as Hsp90, Hsp70 or Hsc70. In this work we show that the level of Sgt1 is increased in HEp-2 cells exposed to heat shock or radicicol. The citrate synthase aggregation assay shows that Sgt1 attenuates aggregation of the enzyme induced by increased temperature as efficiently as p23, a known co-chaperone of Hsp90. We have cloned two fragments of the human Sgt1 gene promoter (−708/+98 and −351/+98) into pGL3-luciferase vector and found that both fragments generated a 2-fold increase in luciferase activity upon heat shock. Furthermore, electrophoretic mobility shift assay revealed binding of the HSF-1 transcription factor to the heat shock element in the proximal (−42/−2) Sgt1 gene promoter fragment. These results indicate that Sgt1 is a co-chaperone protein with an expression pattern matching that of the well known heat shock proteins.

Keywords:

Sgt1, Heat shock, Radicicol, Sgt1 gene promoter, HSF-1, Co-chaperone

Affiliations:
Żabka M.-other affiliation
Leśniak W.-other affiliation
Prus W.-other affiliation
Kuźnicki J.-other affiliation
Filipek A.-Nencki Institute of Experimental Biology, Polish Academy of Sciences (PL)