Institute of Fundamental Technological Research

Background. Blood flow-mediated dilation (FMD) is a noninvasive assessment of vascular endothelial function in humans. The study of the FMD in hypertensive (HT) patients is an important factor supporting the recognition of the early mechanisms of cardiovascular pathologies, and also of the pathogenesis related to hypertension. Objectives. To investigate whether FMD measured on the radial artery (FMD-RA) using high-requency ultrasounds can be used asan alternative to FMD assessed with the lower frequency system onthe brachial artery in patients with HT. Materials and methods. The simultaneous measurements of FMD-RA and FMD measurements in the brachial artery (FMD-BA) were performed on 76 HT patients using 20 MHz and 7–12 MHz linear array probes, and were compared to the FMD measured in healthy groups. All quantitative data are presented as mean ± standard deviation (SD); the p-values of the normality and tests for variables comparisons are listed. The agreement of the FMD-RA and FMD-BA in HT patients was assessed with the Bland–Altman method, and using the intraclass correlation coefficient (ICC). In some statistical calculations, the FMD-RA values were rescaled by dividing them by a factor of 2. Results. The mean FMD-RA and FMD-BA in HT patients were 5.16 ±2.18% (95% confidence interval (95% CI): [4.50%, 5.82%]) and 2.13 ±1.12% (95% CI: [1.76%, 2.49%]), respectively. The FMD-RA and FMD-BA values of HT patients were significantly different than those in respective control groups. The p-values of Mann–Whitney–Wilcoxon tests were less than 0.05. The Bland–Altman coefficient for both measurement methods, FMD-RA and FMD-BA, was 3%, and the ICC was 0.69. Conclusions. Our findings show that FMD-RA, supplementary to FMD-BA measurements, can be used to assess endothelial dysfunction in the group of HT patients. In addition, the FMD-RA measurements met the criteria of high concordance with the FMD-BA measurements.

hypertension, brachial artery, radial artery, endothelial function

A high-frequency scanning system consisting of a 20-MHz linear array transducer combined with a 20-MHz pulsed Dopplerprobe was introduced to evaluate the degree of radial artery flow-mediated dilation (FMD [%]) in two groups of patients after5 min of controlled forearm ischemia followed by reactive hyperemia. In group I, comprising 27 healthy volunteers, FMD (mean ± standard deviation) was 15.26 ± 4.90% (95% confidence interval [CI]: 13.32%–17.20%); in group II, comprising 17 patients with chronic coronary artery disease, FMD was significantly less at 4.53 ± 4.11% (95% CI: 2.42%–6.64%). Specifically, the ratio FMD/SR (mean ± standard deviation),wasequalto5.36×10−4±4.64×10−4 (95%CI:3.54×10−4 to7.18×10−4)ingroupIand1.38×10−4±0.89×10−4 (95% CI: 0.70 × 10−4 to 2.06 × 10−4) in group II. Statistically significant differences between the two groups were confirmed by a Wilcoxon–Mann–Whitney test for both FMD and FMD/SR (p < 0.01). Areas under receiver operating characteristic curves for FMD and FMD/SR were greater than 0.9. The results confirm the usefulness of the proposed measurements of radial artery FMD and SR in differentiation of normal patients from those with chronic coronary artery disease. (E-mail: anowicki@ippt.gov.pl) © 2018 World Federation for Ultrasound in Medicine & Biology. All rights reserved.

Flow-mediated vasodilation, Radial artery, Shear rate, Reactive hyperemia, Endothelium, Pulsed doppler, Ultrasonography

A novel high-frequency scanning system, with a 20-MHz linear array transducer combined with 20-MHz pulsed Doppler, was introduced to evaluate the degree of radial artery flow-mediated dilation (FMD) and shear rate (SR)-normalized FMD (FMD/SR) after 5 min of reactive hyperaemia. In group I, comprising 27 healthy volunteers, FMD was 15 ± 4.8%, and in group II, comprising 17 patients with coronary artery disease, FMD was significantly smaller, being equal to 4.6 ± 4%. FMD/SR was equal to 5.365 ± 4.835·10-4 in group I and 1.3 ± 0.89·10-4 in group II. Statistically significant differences between the two groups were confirmed by Wilcoxon-Mann-Whitney test for FMD and FMD/SR (p-values < 0.01). AUCs of ROC curves for FMD and FMD/SR were greater than 0.9. The results confirm the usefulness of the proposed measurements of radial artery FMD and SR in differentiation of normal subjects from those with atherosclerotic lesions.

flow mediated vasodilation, radial artery, shear rate, reactive hyporaemia, endothelium, pulsed Doppler, ultrasonography.