Partner: K.P. Su

China Medical University Hospital (TW)

Recent publications
1.Kwiatkowski D., Czarny P., Toma M., Jurkowska N., Śliwinska A., Drzewoski J., Bachurska A., Szemraj J., Maes M., Berk M., Su K.P., Gałecki P., Śliwiński T., Associations between DNA Damage, DNA Base Excision Repair Gene Variability and Alzheimer's Disease Risk, DEMENTIA AND GERIATRIC COGNITIVE DISORDERS, ISSN: 1420-8008, DOI: 10.1159/000443953, Vol.41, No.3-4, pp.152-171, 2016
Abstract:

Background: Increased oxidative damage to DNA is one of the pathways involved in Alzheimer's disease (AD). Insufficient base excision repair (BER) is in part responsible for increased oxidative DNA damage. The aim of the present study was to assess the effect of polymorphic variants of BER-involved genes and the peripheral markers of DNA damage and repair in patients with AD. Material and Methods: Comet assays and TaqMan probes were used to assess DNA damage, BER efficiency and polymorphic variants of 12 BER genes in blood samples from 105 AD patients and 130 controls. The DNA repair efficacy (DRE) was calculated according to a specific equation. Results: The levels of endogenous and oxidative DNA damages were higher in AD patients than controls. The polymorphic variants of XRCC1 c.580C>T XRCC1 c.1196A>G and OGG1 c.977C>G are associated with increased DNA damage in AD. Conclusion: Our results show that oxidative stress and disturbances in DRE are particularly responsible for the elevated DNA lesions in AD. The results suggest that oxidative stress and disruption in DNA repair may contribute to increased DNA damage in AD patients and risk of this disease. In addition, disturbances in DRE may be associated with polymorphisms of OGG1 and XRCC1.

Keywords:

DNA damage, DNA base excision repair, Alzheimer's disease risk, Dementia, Oxidative stress

Affiliations:
Kwiatkowski D.-other affiliation
Czarny P.-Medical University of Lodz (PL)
Toma M.-University of Lodz (PL)
Jurkowska N.-University of Lodz (PL)
Śliwinska A.-Medical University of Lodz (PL)
Drzewoski J.-Medical University of Lodz (PL)
Bachurska A.-Medical University of Lodz (PL)
Szemraj J.-Medical University of Lodz (PL)
Maes M.-Deakin University (AU)
Berk M.-Deakin University (AU)
Su K.P.-China Medical University Hospital (TW)
Gałecki P.-Medical University of Lodz (PL)
Śliwiński T.-University of Lodz (PL)
2.Czarny P., Kwiatkowski D., Gałecki P., Talarowska M., Orzechowska A., Bobińska K., Bielecka-Kowalska A., Szemraj J., Maes M., Su K.P., Śliwiński T., Association between single nucleotide polymorphisms of MUTYH, hOGG1 and NEIL1 genes, and depression, JOURNAL OF AFFECTIVE DISORDERS, ISSN: 0165-0327, DOI: 10.1016/j.jad.2015.05.044, Vol.184, pp.90-96, 2015
Abstract:

Background: An elevated levels oxidative modified DNA bases and a decreased efficiency of oxidative DNA damage repair were found in patients with depression disorders, including recurrent type (rDD). The glycosylases are involved in base excision repair (BER), which eliminates oxidative DNA damage. Therefore, we genotyped the single nucleotide polymorphisms (SNPs) of genes encoding three glycosylases: hOGG1, MUTYH and NEILL

Methods: We selected three polymorphisms: c.977C > G - hOGG1 (rs1052133), c.972G > C - MUTYH (rs3219489) and c.*589G > C - NEIL1 (rs4462560). A total of 555 DNA samples (257 cases and 298 controls) were genotyped using TaqMan probes.

Results: The C/C genotype and allele C of the c.*589G > C decreased the risk of rDD occurrence, while the G/G genotype and allele G of the same SNP increased the risk. This polymorphism had a stronger association with early-onset depression (patients with first episode <35 years of age) than with late onset depression (first episode >= 35 years of age). We did not find any significant differences in distribution of alleles and genotypes of other SNPs; however, the G/G genotype of the c.972G > C increased the risk of late onset rDD. We also found that combined genotype C/C-C/C of c.977C > G and c.*589G > C significantly reduced the risk of rDD.

Limitations: Limited sample size and ethnic homogeneity of the studied population.

Conclusion: This is the first study to show that SNPs of genes involved in DNA repair, particularly in BER pathway, may modulate the risk of rDD. These results further support the hypothesis on the involvement of DNA repair mechanisms in pathogenesis of depression.

Keywords:

Depression, Glycosylases, BER, DNA repair, DNA damage

Affiliations:
Czarny P.-Medical University of Lodz (PL)
Kwiatkowski D.-other affiliation
Gałecki P.-Medical University of Lodz (PL)
Talarowska M.-Medical University of Lodz (PL)
Orzechowska A.-other affiliation
Bobińska K.-other affiliation
Bielecka-Kowalska A.-Non-Public Medical Center “Akoria” (PL)
Szemraj J.-Medical University of Lodz (PL)
Maes M.-Deakin University (AU)
Su K.P.-China Medical University Hospital (TW)
Śliwiński T.-University of Lodz (PL)