Partner: Francesca Petronella


Recent publications
1.Zaccagnini F., De Biase D., Bovieri F., Perotto G., Quagliarini E., Bavasso I., Mangino G., Iuliano M., Calogero A., Romeo G., Pratap Singh D., Pierini F., Caracciolo G., Petronella F., De Sio L., Multifunctional FFP2 Face Mask for White Light Disinfection and Pathogens Detection using Hybrid Nanostructures and Optical Metasurfaces, Small, ISSN: 1613-6810, DOI: 10.1002/smll.202400531, pp.2400531-1-15, 2024
Abstract:

A new generation of an FFP2 (Filtering Face Piece of type 2) smart face mask is achieved by integrating broadband hybrid nanomaterials and a self-assembled optical metasurface. The multifunctional FFP2 face mask shows simultaneously white light-assisted on-demand disinfection properties and versatile biosensing capabilities. These properties are achieved by a powerful combination of white light thermoplasmonic responsive hybrid nanomaterials, which provide excellent photo-thermal disinfection properties, and optical metasurface-based colorimetric biosensors, with a very low limit of pathogens detection. The realized system is studied in optical, morphological, spectroscopic, and cell viability assay experiments and environmental monitoring of harmful pathogens, thus highlighting the extraordinary properties in reusability and pathogens detection of the innovative face mask.

Affiliations:
Zaccagnini F.-other affiliation
De Biase D.-other affiliation
Bovieri F.-other affiliation
Perotto G.-other affiliation
Quagliarini E.-other affiliation
Bavasso I.-other affiliation
Mangino G.-other affiliation
Iuliano M.-other affiliation
Calogero A.-Sapienza University of Rome (IT)
Romeo G.-other affiliation
Pratap Singh D.-other affiliation
Pierini F.-IPPT PAN
Caracciolo G.-other affiliation
Petronella F.-other affiliation
De Sio L.-Sapienza University of Rome (IT)
2.Ziai Y., Rinoldi C., Petronella F., Zakrzewska A., De Sio L., Pierini F., Lysozyme-sensitive plasmonic hydrogel nanocomposite for colorimetric dry-eye inflammation biosensing, NANOSCALE, ISSN: 2040-3364, DOI: 10.1039/d4nr01701c, pp.1-11, 2024
Abstract:

Detection of lysozyme levels in ocular fluids is considered crucial for diagnosing and monitoring various health and eye conditions, including dry-eye syndrome. Hydrogel-based nanocomposites have been demonstrated to be one of the most promising platforms for fast and accurate sensing of different biomolecules. In this work, hydrogel, electrospun nanofibers, and plasmonic nanoparticles are combined to fabricate a sensitive and easy-to-use biosensor for lysozyme. Poly(L-lactide-co-caprolactone) (PLCL) nanofibers were covered with silver nanoplates (AgNPls), providing a stable plasmonic platform, where a poly(N-isopropylacrylamide)-based (PNIPAAm) hydrogel layer allows mobility and good integration of the biomolecules. By integrating these components, the platform can also exhibit a colorimetric response to the concentration of lysozyme, allowing for easy and non-invasive monitoring. Quantitative biosensing operates on the principle of localized surface plasmon resonance (LSPR) induced by plasmonic nanoparticles. Chemical, structural, thermal, and optical characterizations were performed on each platform layer, and the platform's ability to detect lysozyme at concentrations relevant to those found in tears of patients with dry-eye syndrome and other related diseases was investigated by colorimetry and UV-Vis spectroscopy. This biosensor's sensitivity and rapid response time, alongside the easy detection by the naked eye, make it a promising tool for early diagnosis and treatment monitoring of eye diseases.

Affiliations:
Ziai Y.-IPPT PAN
Rinoldi C.-IPPT PAN
Petronella F.-other affiliation
Zakrzewska A.-IPPT PAN
De Sio L.-other affiliation
Pierini F.-IPPT PAN
3.Zargarian S., Zakrzewska A., Kosik-Kozioł A., Bartolewska M., Shah S., Li X., Su Q., Petronella F., Marinelli M., De Sio L., Lanzi M., Ding B., Pierini F., Advancing resource sustainability with green photothermal materials: Insights from organic waste-derived and bioderived sources, nanotechnology reviews, ISSN: 2191-9097, DOI: 10.1515/ntrev-2024-0100, Vol.13, No.1, pp.20240100-1-39, 2024
Abstract:

Recently, there has been a surge of interest in developing new types of photothermal materials driven by the ongoing demand for efficient energy conversion, environmental concerns, and the need for sustainable solutions. However, many existing photothermal materials face limitations such as high production costs or narrow absorption bands, hindering their widespread application. In response to these challenges, researchers have redirected their focus toward harnessing the untapped potential of organic waste-derived and bioderived materials. These materials, with photothermal properties derived from their intrinsic composition or transformative processes, offer a sustainable and cost-effective alternative. This review provides an extended categorization of organic waste-derived and bioderived materials based on their origin. Additionally, we investigate the mechanisms underlying the photothermal properties of these materials. Key findings highlight their high photothermal efficiency and versatility in applications such as water and energy harvesting, desalination, biomedical applications, deicing, waste treatment, and environmental remediation. Through their versatile utilization, they demonstrate immense potential in fostering sustainability and support the transition toward a greener and more resilient future. The authors’ perspective on the challenges and potentials of platforms based on these materials is also included, highlighting their immense potential for real-world implementation.

Keywords:

photothermal materials, organic waste valorization, bioderived materials

Affiliations:
Zargarian S.-IPPT PAN
Zakrzewska A.-IPPT PAN
Kosik-Kozioł A.-IPPT PAN
Bartolewska M.-IPPT PAN
Shah S.-IPPT PAN
Li X.-Donghua University (CN)
Su Q.-other affiliation
Petronella F.-other affiliation
Marinelli M.-other affiliation
De Sio L.-other affiliation
Lanzi M.-University of Bologna (IT)
Ding B.-Donghua University (CN)
Pierini F.-IPPT PAN
4.Zakrzewska A., Haghighat Bayan M.A., Nakielski P., Petronella F., De Sio L., Pierini F., Nanotechnology Transition Roadmap toward Multifunctional Stimuli-Responsive Face Masks, ACS Applied Materials and Interfaces, ISSN: 1944-8244, DOI: 10.1021/acsami.2c10335, Vol.14, No.41, pp.46123-46144, 2022
Abstract:

In recent times, the use of personal protective equipment, such as face masks or respirators, is becoming more and more critically important because of common pollution; furthermore, face masks have become a necessary element in the global fight against the COVID-19 pandemic. For this reason, the main mission of scientists has become the development of face masks with exceptional properties that will enhance their performance. The versatility of electrospun polymer nanofibers has determined their suitability as a material for constructing “smart” filter media. This paper provides an overview of the research carried out on nanofibrous filters obtained by electrospinning. The progressive development of the next generation of face masks whose unique properties can be activated in response to a specific external stimulus is highlighted. Thanks to additional components incorporated into the fiber structure, filters can, for example, acquire antibacterial or antiviral properties, self-sterilize the structure, and store the energy generated by users. Despite the discovery of several fascinating possibilities, some of them remain unexplored. Stimuli-responsive filters have the potential to become products of large-scale availability and great importance to society as a whole.

Keywords:

nanostructured face masks, stimuli-responsive nanomaterials, electrospun nanofibers, active filtration, smart filters, COVID-19, antipathogen

Affiliations:
Zakrzewska A.-IPPT PAN
Haghighat Bayan M.A.-IPPT PAN
Nakielski P.-IPPT PAN
Petronella F.-other affiliation
De Sio L.-Sapienza University of Rome (IT)
Pierini F.-IPPT PAN
5.Ziai Y., Petronella F., Rinoldi C., Nakielski P., Zakrzewska A., Kowalewski T.A., Augustyniak W., Li X., Calogero A., Sabała I., Ding B., De Sio L., Pierini F., Chameleon-inspired multifunctional plasmonic nanoplatforms for biosensing applications, NPG Asia Materials, ISSN: 1884-4049, DOI: 10.1038/s41427-022-00365-9, Vol.14, pp.18-1-17, 2022
Abstract:

One of the most fascinating areas in the field of smart biopolymers is biomolecule sensing. Accordingly, multifunctional biomimetic, biocompatible, and stimuli-responsive materials based on hydrogels have attracted much interest. Within this framework, the design of nanostructured materials that do not require any external energy source is beneficial for developing a platform for sensing glucose in body fluids. In this article, we report the realization and application of an innovative platform consisting of two outer layers of a nanocomposite plasmonic hydrogel plus one inner layer of electrospun mat fabricated by electrospinning, where the outer layers exploit photoinitiated free radical polymerization, obtaining a compact and stable device. Inspired by the exceptional features of chameleon skin, plasmonic silver nanocubes are embedded into a poly(N-isopropylacrylamide)-based hydrogel network to obtain enhanced thermoresponsive and antibacterial properties. The introduction of an electrospun mat creates a compatible environment for the homogeneous hydrogel coating while imparting excellent mechanical and structural properties to the final system. Chemical, morphological, and optical characterizations were performed to investigate the structure of the layers and the multifunctional platform. The synergetic effect of the nanostructured system’s photothermal responsivity and antibacterial properties was evaluated. The sensing features associated with the optical properties of silver nanocubes revealed that the proposed multifunctional system is a promising candidate for glucose-sensing applications.

Affiliations:
Ziai Y.-IPPT PAN
Petronella F.-other affiliation
Rinoldi C.-IPPT PAN
Nakielski P.-IPPT PAN
Zakrzewska A.-IPPT PAN
Kowalewski T.A.-IPPT PAN
Augustyniak W.-Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Li X.-Donghua University (CN)
Calogero A.-Sapienza University of Rome (IT)
Sabała I.-Mossakowski Medical Research Centre, Polish Academy of Sciences (PL)
Ding B.-Donghua University (CN)
De Sio L.-Sapienza University of Rome (IT)
Pierini F.-IPPT PAN
6.De Sio L., Ding B., Focsan M., Kogermann K., Pascoal-Faria P., Petronella F., Mitchell G., Zussman E., Pierini F., Personalized reusable face masks with smart nano‐assisted destruction of pathogens for COVID‐19: a visionary road, Chemistry - A European Journal, ISSN: 0947-6539, DOI: 10.1002/chem.202004875, Vol.27, pp.1-20, 2021
Abstract:

The Coronavirus disease 2019 (COVID‐19) emergency has demonstrated that the utilization of face masks plays a critical role in limiting the outbreaks. Healthcare professionals utilize masks all day long without replacing them very frequently, thus representing a source of cross‐infection for patients and themselves. Nanotechnology is a powerful tool with the capability to produce nanomaterials with unique physicochemical and anti‐pathogen properties. Here, we outline how to realize non‐disposable and highly comfortable respirators with light‐triggered self‐disinfection ability by bridging bioactive nanofiber properties and stimuli‐responsive nanomaterials. The visionary road highlighted in this Concept is based on the possibility to develop a new generation of masks based on multifunctional membranes where the presence of nanoclusters and plasmonic nanoparticles arranged in a hierarchical structure enables the realization of a chemically‐driven and on‐demand anti‐pathogen activities. Multilayer electrospun membranes have the ability to dissipate humidity present within the mask, enhancing the wearability and usability. The photo‐thermal disinfected membrane is the core of these 3D printed and reusable masks with moisture pump capability. Personalized face masks with smart nano‐assisted destruction of pathogens will bring enormous advantages to the entire global community, especially for front‐line personnel, and will open up great opportunities for innovative medical applications.

Keywords:

face masks, light-responsive nanomaterials, anti-pathogen, electrospinning, digitally personalized

Affiliations:
De Sio L.-Sapienza University of Rome (IT)
Ding B.-Donghua University (CN)
Focsan M.-other affiliation
Kogermann K.-other affiliation
Pascoal-Faria P.-other affiliation
Petronella F.-other affiliation
Mitchell G.-other affiliation
Zussman E.-Technion-Israel Institute of Technology (IL)
Pierini F.-IPPT PAN
7.Rinoldi C., Zargarian S.S., Nakielski P., Li X., Liguori A., Petronella F., Presutti D., Wang Q., Costantini M., De Sio L., Gualandi C., Ding B., Pierini F., Nanotechnology-assisted RNA delivery: from nucleic acid therapeutics to COVID-19 vaccines, Small Methods, ISSN: 2366-9608, DOI: 10.1002/smtd.202100402, Vol.5, No.9, pp.2100402-1-49, 2021
Abstract:

In recent years, the main quest of science has been the pioneering of the groundbreaking biomedical strategies needed for achieving a personalized medicine. Ribonucleic acids (RNAs) are outstanding bioactive macromolecules identified as pivotal actors in regulating a wide range of biochemical pathways. The ability to intimately control the cell fate and tissue activities makes RNA-based drugs the most fascinating family of bioactive agents. However, achieving a widespread application of RNA therapeutics in humans is still a challenging feat, due to both the instability of naked RNA and the presence of biological barriers aimed at hindering the entrance of RNA into cells. Recently, material scientists’ enormous efforts have led to the development of various classes of nanostructured carriers customized to overcome these limitations. This work systematically reviews the current advances in developing the next generation of drugs based on nanotechnology-assisted RNA delivery. The features of the most used RNA molecules are presented, together with the development strategies and properties of nanostructured vehicles. Also provided is an in-depth overview of various therapeutic applications of the presented systems, including coronavirus disease vaccines and the newest trends in the field. Lastly, emerging challenges and future perspectives for nanotechnology-mediated RNA therapies are discussed.

Affiliations:
Rinoldi C.-IPPT PAN
Zargarian S.S.-IPPT PAN
Nakielski P.-IPPT PAN
Li X.-Donghua University (CN)
Liguori A.-University of Bologna (IT)
Petronella F.-other affiliation
Presutti D.-Institute of Physical Chemistry, Polish Academy of Sciences (PL)
Wang Q.-Donghua University (CN)
Costantini M.-Sapienza University of Rome (IT)
De Sio L.-Sapienza University of Rome (IT)
Gualandi C.-University of Bologna (IT)
Ding B.-Donghua University (CN)
Pierini F.-IPPT PAN

List of chapters in recent monographs
1.
720
Petronella F., Stoia D., Ziai Y., Zaccagnini F., Scognamiglio V., Maniu D., Rinoldi C., Focsan M., Antonacci A., Pierini F., De Sio L., Novel Optical Materials, rozdział: Chapter 6: Plasmonic-based Biosensors for the Rapid Detection of Harmful Pathogens, World Scientific, 1, pp.155-194, 2023

Conference abstracts
1.Ziai Y., Petronella F., Rinoldi C., Nakielski P., De Sio L., Pierini F., An AgNPs-incorporated hydrogel-based nanocomposite for lysozyme biosensing, NANOMAT2023, 6th International Conference on Functional Nanomaterials and Nanodevices, 2023-08-27/08-30, Warsaw (PL), No.075, pp.109, 2023
Abstract:

Lysozyme, an enzyme found in various bodily fluids, holds immense importance as a biomolecule with numerous diagnostic implications. In the realm of ophthalmology, lysozyme detection in tears emerges as a precious tool for identifying and addressing dry and inflamed eyes. To enhance the precision and efficiency of lysozyme detection, Smart materials, such as hydrogels and electrospun nanofibers, have been confirmed to be promising candidates for sensing platforms. Plasmonic nanoparticles, on the other hand, offer enhanced optical properties that allow for localized surface plasmon resonance (LSPR), which has been used alongside these substrates. By integrating these smart materials into biosensing platforms, researchers can achieve rapid, reliable, and non-invasive lysozyme detection from tears.
To achieve this goal, a layered platform consisting of a hydrogel layer, electrospun nanofibers, and plasmonic nanoparticles was designed and fabricated. Electrospun mat of poly (L-lactide-co-caprolactone) (PLCL) was used as the support, providing suitable mechanical properties to the platform. Silver nanoplates were immobilized on top of the electrospun nanofibers, where a layer of poly(N-isopropylacrylamide)-based hydrogel was added. With its porous 3D structure and high water content, the hydrogel network allows enhancement in photothermal responsivity. Moreover, due to its fluid nature, the maneuvering of the biomolecules is much easier, making the biosensing procedure more accurate. The structure of each layer, their cross-section, and the whole platform were investigated chemically, morphologically, and optically. The fast photothermal responsitivity of the platform and sensing features were studied, revealing the applicability of the system as a biosensor for detecting lysozyme.

Affiliations:
Ziai Y.-IPPT PAN
Petronella F.-other affiliation
Rinoldi C.-IPPT PAN
Nakielski P.-IPPT PAN
De Sio L.-Sapienza University of Rome (IT)
Pierini F.-IPPT PAN