dr inż. Zuzanna Krysiak

Zakład Biosystemów i Miękkiej Materii (ZBiMM)
Pracownia Zastosowań Nanomateriałów Polimerowych (PZNP)
stanowisko: adiunkt
telefon: (+48) 22 826 12 81 wew.: 448
pokój: 321
e-mail: zkrysiak

Doktorat
2022-10-24Designing and characterization of electrospun patches for atopic skin  (Akademia Górniczo-Hutnicza w Krakowie)
promotor -- dr hab. inż. Urszula Stachewicz, Akademia Górniczo-Hutnicza w Krakowie
1548 
Ostatnie publikacje
1.Haghighat Bayan Mohammad A., Kosik-Kozioł A., Krysiak Zuzanna J., Zakrzewska A., Lanzi M., Nakielski P., Pierini F., Gold Nanostar-Decorated Electrospun Nanofibers Enable On-Demand Drug Delivery, Macromolecular Rapid Communications, ISSN: 1022-1336, DOI: 10.1002/marc.202500033, pp.2500033-1-10, 2025

Streszczenie:

This study explores the development of a photo-responsive bicomponent electrospun platform and its drug delivery capabilities. This platform is composed of two polymers of poly(lactide-co-glycolide) (PLGA) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). Then, the platform is decorated with plasmonic gold nanostars (Au NSs) that are capable of on-demand drug release. Using Rhodamine-B (RhB) as a model drug, the drug release behavior of the bi-polymer system is compared versus homopolymer fibers. The RhB is incorporated in the PHBV part of the platform, which provides a more sustained drug release, both in the absence and presence of near-infrared (NIR) irradiation. Under NIR exposure, thermal imaging reveals a notable increase in surface temperature, facilitating enhanced drug release. Furthermore, the platform demonstrates on-demand drug release upon multiple NIR irradiation cycles. This platform offers a promising approach for stimuli-responsive drug delivery, making it a strong candidate for on-demand therapy applications.

Afiliacje autorów:

Haghighat Bayan Mohammad A.-IPPT PAN
Kosik-Kozioł A.-IPPT PAN
Krysiak Zuzanna J.-IPPT PAN
Zakrzewska A.-IPPT PAN
Lanzi M.-University of Bologna (IT)
Nakielski P.-IPPT PAN
Pierini F.-IPPT PAN
100p.
2.Krysiak Z., Rybak D., Kurniawan T., Zakrzewska A., Pierini F., Light-Driven Structural Detachment and Controlled Release in Smart Antibacterial Multilayer Platforms, Macromolecular Materials and Engineering, ISSN: 1438-7492, DOI: 10.1002/mame.202400462, pp.2400462-1-9, 2025

Streszczenie:

Smart materials, especially light-responsive, have become a key research area due to their tunable properties. It is related to the ability to undergo physical or chemical changes in response to external stimuli. Among them, photothermal responsive materials have attracted great interest. This study focuses on the development of a multilayer system (MS) consisting of benzophenone-modified polydimethylsiloxane (PDMS) ring and a thermo-responsive core made of poly(N-isopropylacrylamide-co-N-isopropylomethacrylamide) (P(NIPAAm-co-NIPMAAm)), gelatin, and gelatin methacrylate (GelMA). The system utilizes the thermal sensitivity of P(NIPAAm-co-NIPMAAm) and the photothermal effect of gold nanorods (AuNRs) to achieve an on-demand controlled release mechanism within 6 min of near-infrared (NIR) light irradiation. The mechanical properties investigated in the compression test show significant improvement in MS, reaching 60 times greater value than the material without a PDMS ring. In addition, NIR irradiation for 15 min activated the antimicrobial properties, eliminating 99.9% of E. Coli and 100% of S. Aureus, thus presenting pathogen eradication. This platform provides a versatile methodology for developing next-generation smart materials, advanced delivery mechanisms, and multifunctional nanostructured composites. This work highlights the potential of photosensitive materials to revolutionize the field of soft robotics, optics and actuators, and on-demand systems by providing precise control over release dynamics and improved material properties.

Afiliacje autorów:

Krysiak Z.-IPPT PAN
Rybak D.-IPPT PAN
Kurniawan T.-IPPT PAN
Zakrzewska A.-IPPT PAN
Pierini F.-IPPT PAN
70p.
3.Bartolewska M., Kosik-Kozioł A., Korwek Z., Krysiak Z., Devis M., Mazur M., Giuseppe F., Pierini F., Eumelanin-Enhanced Photothermal Disinfection of Contact Lenses Using a Sustainable Marine Nanoplatform Engineered with Electrospun Nanofibers, ADVANCED HEALTHCARE MATERIALS, ISSN: 2192-2659, DOI: 10.1002/adhm.202402431, pp.2402431-1-21, 2024

Streszczenie:

Bacterial keratitis (BK) is a severe eye infection commonly associated with Staphylococcus aureus (S. aureus), posing a significant risk to vision, especially among contact lens wearers. This research introduces a novel smart nanoplatform (deMS@cNF), developed from demineralized mussel shells (deMS) and reinforced with chitin (CT) nanofibrils, specifically designed for portable photothermal disinfection of contact lenses. The nanoplatform leverages the photothermal properties of eumelanin in mussel shells (MS), which, when activated by a simple bike flashlight, rapidly heats to temperatures up to 95 °C, effectively destroying bacterial contamination. In vitro tests demonstrate that the nanoplatform is biocompatible and non-toxic, making it suitable for medical applications. This study highlights an innovative approach to converting marine biowaste into a safe, effective, and low-cost portable method for disinfecting contact lenses, showcasing the potential of the deMS@cNF platform for broader antimicrobial applications.

Afiliacje autorów:

Bartolewska M.-IPPT PAN
Kosik-Kozioł A.-IPPT PAN
Korwek Z.-IPPT PAN
Krysiak Z.-IPPT PAN
Devis M.-other affiliation
Mazur M.-other affiliation
Giuseppe F.-other affiliation
Pierini F.-IPPT PAN
140p.
4.Haghighat Bayan M.A., Rinoldi C., Kosik-Kozioł A., Bartolewska M., Rybak D., Zargarian S., Shah S., Krysiak Z., Zhang S., Lanzi M., Nakielski P., Ding B., Pierini F., Solar-to-NIR Light Activable PHBV/ICG Nanofiber-Based Face Masks with On-Demand Combined Photothermal and Photodynamic Antibacterial Properties, Advanced Materials Technologies, ISSN: 2365-709X, DOI: 10.1002/admt.202400450, pp.2400450-1-18, 2024

Streszczenie:

Hierarchical nanostructures fabricate by electrospinning in combination with light-responsive agents offer promising scenarios for developing novel activable antibacterial interfaces. This study introduces an innovative antibacterial face mask developed from poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibers integrated with indocyanine green (ICG), targeting the urgent need for effective antimicrobial protection for community health workers. The research focuses on fabricating and characterizing this nanofibrous material, evaluating the mask's mechanical and chemical properties, investigating its particle filtration, and assessing antibacterial efficacy under photothermal conditions for reactive oxygen species (ROS) generation. The PHBV/ICG nanofibers are produced using an electrospinning process, and the nanofibrous construct's morphology, structure, and photothermal response are investigated. The antibacterial efficacy of the nanofibers is tested, and substantial bacterial inactivation under both near-infrared (NIR) and solar irradiation is demonstrated due to the photothermal response of the nanofibers. The material's photothermal response is further analyzed under cyclic irradiation to simulate real-world conditions, confirming its durability and consistency. This study highlights the synergistic impact of PHBV and ICG in enhancing antibacterial activity, presenting a biocompatible and environmentally friendly solution. These findings offer a promising path for developing innovative face masks that contribute significantly to the field of antibacterial materials and solve critical public health challenges.

Afiliacje autorów:

Haghighat Bayan M.A.-IPPT PAN
Rinoldi C.-IPPT PAN
Kosik-Kozioł A.-IPPT PAN
Bartolewska M.-IPPT PAN
Rybak D.-IPPT PAN
Zargarian S.-IPPT PAN
Shah S.-IPPT PAN
Krysiak Z.-IPPT PAN
Zhang S.-other affiliation
Lanzi M.-University of Bologna (IT)
Nakielski P.-IPPT PAN
Ding B.-Donghua University (CN)
Pierini F.-IPPT PAN
100p.
5.Korszun‑Karbowniczak J., Krysiak Z., Saluk J., Niemcewicz M., Zdanowski R., The Progress in Molecular Transport and Therapeutic Development in Human Blood–Brain Barrier Models in Neurological Disorders, Cellular and Molecular Neurobiology, ISSN: 0272-4340, DOI: 10.1007/s10571-024-01473-6, Vol.44, pp.34-1-15, 2024

Streszczenie:

The blood–brain barrier (BBB) is responsible for maintaining homeostasis within the central nervous system (CNS). Depending on its permeability, certain substances can penetrate the brain, while others are restricted in their passage. Therefore, the knowledge about BBB structure and function is essential for understanding physiological and pathological brain processes. Consequently, the functional models can serve as a key to help reveal this unknown. There are many in vitro models available to study molecular mechanisms that occur in the barrier. Brain endothelial cells grown in culture are commonly used to modeling the BBB. Current BBB platforms include: monolayer platforms, transwell, matrigel, spheroidal, and tissue-on-chip models. In this paper, the BBB structure, molecular characteristic, as well as its dysfunctions as a consequence of aging, neurodegeneration, or under hypoxia and neurotoxic conditions are presented. Furthermore, the current modelling strategies that can be used to study BBB for the purpose of further drugs development that may reach CNS are also described.

Słowa kluczowe:

Blood-brain barrier (BBB), Hypoxia, BBB permeability, Tight junctions

Afiliacje autorów:

Korszun‑Karbowniczak J.-other affiliation
Krysiak Z.-other affiliation
Saluk J.-other affiliation
Niemcewicz M.-other affiliation
Zdanowski R.-other affiliation
100p.
6.Szewczyk P., Kopacz M., Krysiak Z., Stachewicz U., Oil-Infused Polymer Fiber Membranes as Porous Patches for Long-Term Skin Hydration and Moisturization, Macromolecular Materials and Engineering, ISSN: 1438-7492, DOI: 10.1002/mame.202300291, Vol.309, No.2, pp.2300291-1-8, 2024

Streszczenie:

Skin allergies and diseases, including atopic dermatitis (AD), affect millions worldwide. Current treatments for AD are often expensive, leading to a need for cost-effective solutions. Here, using fiber-based patches to maintain and increase skin hydration is explored, which helps treat eczema and AD. Nanofiber membranes are manufactured via electrospinning of eight different polymers: nylon 6 (PA6), polyimide (PI), poly(3-hydroxybuty-rate-co-3-hydroxyvalerate (PHBV), poly(l-lactide) (PLLA), polycaprolactone (PCL), and polystyrene (PS), and two molecular weights poly(vinyl butyral-co-vinyl alcohol-co-vinyl acetate) (PVB). Further, their morphology is examined through scanning electron microscopy (SEM), fibers, and pores diameter, wettability, and membrane thickness. Additionally, water vapor transmission rates (WVTR) are measured, and notably, skin hydration tests are conducted before and after using evening primrose oil-infused patches. The comparison and findings highlight the flexibility of electrospun patches, demonstrating their potential in maintaining skin hydration for 6 h and enhancing skin moisture, which are necessary in AD treatment. These insights, which focus on selecting the most effective performance patches, help improve skin moisture, leading to tailored treatments for AD, which can significantly impact the efforts to reduce healthcare costs and simplify skincare steps.

Afiliacje autorów:

Szewczyk P.-other affiliation
Kopacz M.-other affiliation
Krysiak Z.-other affiliation
Stachewicz U.-AGH University of Science and Technology (PL)
70p.
7.Krysiak Z., Stachewicz U., Electrospun fibers as carriers for topical drug delivery and release in skin bandages and patches for atopic dermatitis treatment, WIREs Nanomedicine and Nanobiotechnology, ISSN: 1939-0041, DOI: 10.1002/wnan.1829, Vol.15, No.1, pp.e1829-1-35, 2023

Streszczenie:

The skin is a complex layer system and the most important barrier between the environment and the organism. In this review, we describe some widespread skin problems, with a focus on eczema, which are affecting more and more people all over the world. Most of treatment methods for atopic dermatitis (AD) are focused on increasing skin moisture and protecting from bacterial infection and external irritation. Topical and transdermal treatments have specific requirements for drug delivery. Breathability, flexibility, good mechanical properties, biocompatibility, and efficacy are important for the patches used for skin. Up to today, electrospun fibers are mostly used for wound dressing. Their properties, however, meet the requirements for skin patches for the treatment of AD. Active agents can be incorporated into fibers by blending, coaxial or side-by-side electrospinning, and also by physical absorption post-processing. Drug release from the electrospun membranes is affected by drug and polymer properties and the technique used to combine them into the patch. We describe in detail the in vitro release mechanisms, parameters affecting the drug transport, and their kinetics, including theoretical approaches. In addition, we present the current research on skin patch design. This review summarizes the current extensive know-how on electrospun fibers as skin drug delivery systems, while underlining the advantages in their prospective use as patches for atopic dermatitis.

This article is categorized under:

Implantable Materials and Surgical Technologies > Nanomaterials and Implants
Implantable Materials and Surgical Technologies > Nanotechnology in Tissue Repair and Replacement
Therapeutic Approaches and Drug Discovery > Emerging Technologies

Słowa kluczowe:

atopic dermatitis, drug delivery, electrospinning, electrospun fibers, release, skin patches

Afiliacje autorów:

Krysiak Z.-other affiliation
Stachewicz U.-AGH University of Science and Technology (PL)
140p.
8.Wasyłeczko M., Krysiak Z.J., Łukowska E., Gruba M., Sikorska W., Kruk A., Dulnik J., Czubak J., Chwojnowski A., Three-dimensional scaffolds for bioengineering of cartilage tissue, Biocybernetics and Biomedical Engineering, ISSN: 0208-5216, DOI: 10.1016/j.bbe.2022.03.004, Vol.42, No.2, pp.494-511, 2022

Streszczenie:

The cartilage tissue is neither supplied with blood nor innervated, so it cannot heal by itself. Thus, its reconstruction is highly challenging and requires external support. Cartilage diseases are becoming more common due to the aging population and obesity. Among young people, it is usually a post-traumatic complication. Slight cartilage damage leads to the spontaneous formation of fibrous tissue, not resistant to abrasion and stress, resulting in cartilage degradation and the progression of the disease. For these reasons, cartilage regeneration requires further research, including use of new type of biomaterials for scaffolds. This paper shows cartilage characteristics within its most frequent problems and treatment strategies, including a promising method that combines scaffolds and human cells. Structure and material requirements, manufacturing methods, and commercially available scaffolds were described. Also, the comparison of poly(L-lactide) (PLLA) and polyethersulfone (PES) 3D membranes obtained by a phase inversion method using nonwovens as a pore-forming additives were reported. The scaffolds' structure and the growth ability of human chondrocytes were compared. Scaffolds' structure, cells morphology, and protein presence in the membranes were examined with a scanning electron microscope. The metabolic activity of cells was tested with the MTT assay. The structure of the scaffolds and the growth capacity of human chondrocytes were compared. Obtained results showed higher cell activity and protein content for PES scaffolds than for PLLA. The PES membrane had better mechanical properties (e.g. ripping), greater chondrocytes proliferation, and thus a better secretion of proteins which build up the cartilage structure.

Słowa kluczowe:

3D-scaffolds, membrane structure, polyethersulfone, poly(L-lactide), chondrocyte culture, cartilage regeneration

Afiliacje autorów:

Wasyłeczko M.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Krysiak Z.J.-other affiliation
Łukowska E.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Gruba M.-Gruca Orthopedic and Trauma Teaching Hospital, Centre of Postgraduate Medical Education (PL)
Sikorska W.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
Kruk A.-Politechnika Warszawska (PL)
Dulnik J.-IPPT PAN
Czubak J.-Gruca Orthopedic and Trauma Teaching Hospital, Centre of Postgraduate Medical Education (PL)
Chwojnowski A.-Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences (PL)
140p.
9.Krysiak Z., Abdolmaleki H., Agarwala S., Stachewicz U., Inkjet Printing of Electrodes on Electrospun Micro- and Nanofiber Hydrophobic Membranes for Flexible and Smart Textile Applications, Polymers, ISSN: 2073-4360, DOI: 10.3390/polym14225043, Vol.14, No.22, pp.5043-1-14, 2022

Streszczenie:

With the increasing demand for smart textile and sensor applications, the interest in printed electronics is rising. In this study, we explore the applicability of electrospun membranes, characterized by high porosity and hydrophobicity, as potential substrates for printed electronics. The two most common inks, silver and carbon, were used in inkjet printing to create a conductive paths on electrospun membranes. As substrates, we selected hydrophobic polymers, such as polyimide (PI), low- and high-molecular-weight poly (vinyl butyral-co-vinyl alcohol-co-vinyl acetate) (PVB) and polystyrene (PS). Electrospinning of PI and PVB resulted in nanofibers in the range of 300–500 nm and PVB and PS microfibers (1–5 μm). The printed patterns were investigated with a scanning electron microscope (SEM) and resistance measurements. To verify the biocompatibility of printed electrodes on the membranes, an indirect cytotoxicity test with cells (MG-63) was performed. In this research, we demonstrated good printability of silver and carbon inks on flexible PI, PVB and PS electrospun membranes, leading to electrodes with excellent conductivity. The cytotoxicity study indicated the possibility of using manufactured printed electronics for various sensors and also as topical wearable devices.

Słowa kluczowe:

printed electronics,inkjet printing,electrospinning,fibers,hydrophobicity,cells,membrane

Afiliacje autorów:

Krysiak Z.-other affiliation
Abdolmaleki H.-other affiliation
Agarwala S.-other affiliation
Stachewicz U.-AGH University of Science and Technology (PL)
100p.
10.Krysiak Z., Stachewicz U., Urea-Based Patches with Controlled Release for Potential Atopic Dermatitis Treatment, Pharmaceutics, ISSN: 1999-4923, DOI: 10.3390/pharmaceutics14071494, Vol.14, No.7, pp.1494-1-10, 2022

Streszczenie:

Skin diseases such as atopic dermatitis (AD) are widespread and affect people all over the world. Current treatments for dry and itchy skin are mostly focused on pharmaceutical solutions, while supportive therapies such as ointments bring immediate relief. Electrospun membranes are commonly used as a drug delivery system, as they have a high surface to volume area, resulting in high loading capacity. Within this study we present the manufacturing strategies of skin patches using polymer membranes with active substances for treating various skin problems. Here, we manufactured the skin patches using electrospun poly(vinyl butyral-co-vinyl alcohol-co-vinyl acetate) (PVB) fibers blended and electrosprayed with urea. The highest cumulative release of urea was obtained from the PVB patches manufactured via blend electrospinning with 5% of the urea incorporated in the fiber. The maximum concentration of released urea was acquired after 30 min, which was followed up by 6 h of constant release level. The simultaneous electrospinning and electrospraying limited the urea deposition and resulted in the lowest urea incorporation followed by the low release level. The urea-based patches, manufactured via blend electrospinning, exhibited a great potential as overnight treatment for various skin problems and their development can bring new trends to the textile-based therapies for AD.

Słowa kluczowe:

PVB, electrospinning, electrospray, fibers, urea

Afiliacje autorów:

Krysiak Z.-other affiliation
Stachewicz U.-AGH University of Science and Technology (PL)
100p.
11.Krysiak Z., Szewczyk P., Berniak K., Sroczyk E., Boratyn E., Stachewicz U., Stretchable skin hydrating PVB patches with controlled pores' size and shape for deliberate evening primrose oil spreading, transport and release, , DOI: 10.1016/j.bioadv.2022.212786, Vol.136, pp.212786-1-14, 2022

Streszczenie:

With the increasing number of skin problems such as atopic dermatitis and the number of affected people, scientists are looking for alternative treatments to standard ointment or cream applications. Electrospun membranes are known for their high porosity and surface to volume area, which leads to a great loading capacity and their applications as skin patches. Polymer fibers are widely used for biomedical applications such as drug delivery systems or regenerative medicine. Importantly, fibrous meshes are used as oil reservoirs due to their excellent absorption properties. In our study, nano- and microfibers of poly (vinyl butyral-co-vinyl alcohol-co-vinyl acetate) (PVB) were electrospun. The biocompatibility of PVB fibers was confirmed with the keratinocytes culture studies, including cells' proliferation and replication tests. To verify the usability and stretchability of electrospun membranes, they were tested in two forms as-spun and elongated after uniaxially stretched. We examine oil transport through the patches for as-spun fibers and compare it with the numerical simulation of oil flow in the 3D reconstruction of nano- and microfiber networks. Evening primrose oil spreading and water vapor transmission rate (WVTR) tests were performed too. Finally, for skin hydration tests, manufactured materials loaded with evening primrose oil were applied to the forearm of volunteers for 6 h, showing increased skin moisture after using patches. This study clearly demonstrates that pore size and shape, together with fiber diameter, influence oil transport in the electrospun patches allowing to understand the key driving process of electrospun PVB patches for skin hydration applications. The oil release improves skin moisture and can be designed regarding the needs, by manufacturing different fibers' sizes and arrangements. The fibrous based patches loaded with oils are easy to handle and could remain on the altered skin for a long time and deliver the oil, therefore they are an ideal material for overnight bandages for skin treatment.

Słowa kluczowe:

Controlled oil spreading, Electrospun fibers, fiber elongation, Atopic skin, Keratinocytes, Oil flow numerical modeling

Afiliacje autorów:

Krysiak Z.-other affiliation
Szewczyk P.-other affiliation
Berniak K.-other affiliation
Sroczyk E.-other affiliation
Boratyn E.-other affiliation
Stachewicz U.-AGH University of Science and Technology (PL)
12.Kaniuk Ł., Ferraris S., Spriano S., Luxbacher T., Krysiak Z., Berniak K., Zaszczyńska A., Marzec M.M., Bernasik A., Sajkiewicz P., Stachewicz U., Time-dependent effects on physicochemical and surface properties of PHBV fibers and films in relation to their interactions with fibroblasts, APPLIED SURFACE SCIENCE, ISSN: 0169-4332, DOI: 10.1016/j.apsusc.2021.148983, Vol.545, pp.148983-1-13, 2021

Streszczenie:

Biodegradability or materials physicochemical stability are the key biomaterials selection parameters for various medical and tissue engineering applications. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is a natural copolymer known from its biocompatibility with great support for cells growth and attachment on films and fibers. In our studies, the physicochemical properties of electrospun PHBV fibers and spin-coated films aged for 1, 4 and 8 weeks were analyzed using bulk (FTIR) and surface chemistry (XPS) methods and water contact angle. Further, we characterized the zeta potential changes after aging, by means of electrokinetic measurements, and cell responses to it, using NIH 3T3 murine fibroblasts. Colorimetric MTS cell viability test allowed the assessment of cell proliferation. Additionally, the morphology of fibroblasts and biointerfaces were studied by confocal laser and electron scanning microscopy (CLSM and SEM). These studies indicated that the activity, attachment and proliferation of fibroblasts is independent of aging of PHBV fibers and films. PHBV films show very stable zeta potential over 8 weeks of aging, opposite to PHBV fibers. Importantly, the flat film of PHBV increases cell proliferation, while the fibrous meshes are an excellent support for their stretching. The results of the study revealed clear advantages of PHBV films and fibrous meshes in cell-material interaction.

Słowa kluczowe:

cell morphology, fibroblast, electrospun fibers, PHBV, Zeta potential

Afiliacje autorów:

Kaniuk Ł.-other affiliation
Ferraris S.-other affiliation
Spriano S.-other affiliation
Luxbacher T.-other affiliation
Krysiak Z.-other affiliation
Berniak K.-other affiliation
Zaszczyńska A.-IPPT PAN
Marzec M.M.-other affiliation
Bernasik A.-other affiliation
Sajkiewicz P.-IPPT PAN
Stachewicz U.-AGH University of Science and Technology (PL)
140p.
13.Krysiak Z., Knapczyk-Korczak J., Gabriela M., Stachewicz U., Moisturizing effect of skin patches with hydrophobic and hydrophilic electrospun fibers for atopic dermatitis, COLLOIDS AND SURFACES B-BIOINTERFACES, ISSN: 0927-7765, DOI: 10.1016/j.colsurfb.2020.111554, Vol.199, pp.111554-1-8, 2021

Streszczenie:

Atopic dermatitis (eczema), one of the most common disease and also most difficult to treat, is seeking for novel development not only in medicine but also in bioengineering. Moisturization is the key in eczema treatment as dry skin triggers inflammation that damages the skin barrier. Thus, here we combine electrospun hydrophobic polystyrene (PS) and hydrophilic nylon 6 (PA6) with oils to create patches helping to moisturize atopic skin. The fibrous membranes manufactured using electrospinning: PS, PA6, composite PS – PA6 and sandwich system combining them were characterized by water vapor transmission rates (WVTR) and fluid uptake ability (FUA). To create the most effective moisturizing patches we use borage, black cumin seed and evening primrose oil and tested their spreading. We show a great potential of our designed patches, the oil release tests on a skin and their moisturizing effect were verified. Our results distinctly reveal that both fiber sizes and hydrophilicity/hydrophobicity of polymer influence oil spreading, release from membranes and WVTR measurements. Importantly, the direct skin test indicates the evident increase of hydration for both dry and normal skin after using the patches. The electrospun patches based on the hydrophobic and hydrophilic polymers have outstanding properties to be used as oil carriers for atopic dermatitis treatment.

Słowa kluczowe:

PS – PA6 composite, Electrospinning, Skin patches, Oil carriers, Atopic skin, Controlled oil release

Afiliacje autorów:

Krysiak Z.-other affiliation
Knapczyk-Korczak J.-other affiliation
Gabriela M.-other affiliation
Stachewicz U.-AGH University of Science and Technology (PL)
100p.
14.Sara M., Ura D., Krysiak Z., Kaniuk Ł., Szewczyk P., Stachewicz U., Electrospun PCL Patches with Controlled Fiber Morphology and Mechanical Performance for Skin Moisturization via Long-Term Release of Hemp Oil for Atopic Dermatitis, Membranes, ISSN: 2077-0375, DOI: 10.3390/membranes11010026, Vol.11, No.1, pp.26-1-13, 2021

Streszczenie:

Atopic dermatitis (AD) is a chronic, inflammatory skin condition, caused by wide genetic, environmental, or immunologic factors. AD is very common in children but can occur at any age. The lack of long-term treatments forces the development of new strategies for skin regeneration. Polycaprolactone (PCL) is a well-developed, tissue-compatible biomaterial showing also good mechanical properties. In our study, we designed the electrospun PCL patches with controlled architecture and topography for long-term release in time. Hemp oil shows anti-inflammatory and antibacterial properties, increasing also the skin moisture without clogging the pores. It can be used as an alternative cure for patients that do not respond to traditional treatments. In the study, we tested the mechanical properties of PCL fibers, and the hemp oil spreading together with the release in time measured on skin model and human skin. The PCL membranes are suitable material as patches or bandages, characterized by good mechanical properties and high permeability. Importantly, PCL patches showed release of hemp oil up to 55% within 6 h, increasing also the skin moisture up to 25%. Our results confirmed that electrospun PCL patches are great material as oil carriers indicating a high potential to be used as skin patches for AD skin treatment.

Słowa kluczowe:

PCL, electrospinning, fibers, tensile strength, hemp oil, skin patches, release, skin moisture, atopic dermatitis

Afiliacje autorów:

Sara M.-other affiliation
Ura D.-other affiliation
Krysiak Z.-IPPT PAN
Kaniuk Ł.-other affiliation
Szewczyk P.-other affiliation
Stachewicz U.-AGH University of Science and Technology (PL)
15.Krysiak Z., Gawlik M., Knapczyk-Korczak J., Kaniuk Ł., Stachewicz U., Hierarchical Composite Meshes of Electrospun PS Microfibers with PA6 Nanofibers for Regenerative Medicine, Materials, ISSN: 1996-1944, DOI: 10.3390/ma13081974, Vol.13, No.8, pp.1974-1-11, 2020

Streszczenie:

One of the most frequently applied polymers in regenerative medicine is polystyrene (PS), which is commonly used as a flat surface and requires surface modifications for cell culture study. Here, hierarchical composite meshes were fabricated via electrospinning PS with nylon 6 (PA6) to obtain enhanced cell proliferation, development, and integration with nondegradable polymer fibers. The biomimetic approach of designed meshes was verified with a scanning electron microscope (SEM) and MTS assay up to 7 days of cell culture. In particular, adding PA6 nanofibers changes the fibroblast attachment to meshes and their development, which can be observed by cell flattening, filopodia formation, and spreading. The proposed single-step manufacturing of meshes controlled the surface properties and roughness of produced composites, allowing governing cell behavior. Within this study, we show the alternative engineering of nondegradable meshes without post-treatment steps, which can be used in various applications in regenerative medicine.

Słowa kluczowe:

polystyrene, nylon 6, electrospun fibers, composite mesh, proliferation, roughness

Afiliacje autorów:

Krysiak Z.-other affiliation
Gawlik M.-other affiliation
Knapczyk-Korczak J.-other affiliation
Kaniuk Ł.-other affiliation
Stachewicz U.-AGH University of Science and Technology (PL)
140p.
16.Kaniuk Ł., Krysiak Z., Sara M., Stachewicz U., Osteoblasts and fibroblasts attachment to poly(3-hydroxybutyric acid-co-3-hydrovaleric acid) (PHBV) film and electrospun scaffolds, Materials Science and Engineering C, ISSN: 0928-4931, DOI: 10.1016/j.msec.2020.110668, Vol.110, pp.110668-1-8, 2020

Streszczenie:

The cellular response is the most crucial in vitro research. Materials' biocompatibility is determined based on cell proliferation and growth. Moreover, the topography of the scaffold surface is the key to enhance cell attachment and anchoring that importantly control further tissue development. Individual cell types have specific preferences regarding the type of surface and its geometry. In our research, we used poly(3-hydroxybutyric acid-co-3-hydrovaleric acid) PHBV to produce two types of substrate: a 3D structure of electrospun fibers and 2D flat films. The PHBV products were morphologically characterized by scanning electron microscopy (SEM). The cytocompatibility was evaluated with cell viability and proliferation using two different types of cells: human osteoblast-like cells (MG-63) and NIH 3 T3 murine fibroblast cells. The behaviour of both cell types was compared on the similar PHBV fiber scaffolds and films using two types of polystyrene (PS) based substrate for the cell culture study: unmodified PS that is not favourable for the attachment of cells and on tissue culture polystyrene (TCPS) plates, which are chemically modify to enhance cells attachment. The results clearly showed high biocompatibility of PHBV as both types of cells showed similar proliferation. These results indicated that PHBV scaffolds are suitable for the development of multifunctional substrates facilitating the growth of different types of tissue regardless of the 3D and 2D designed structures for regeneration purposes.

Słowa kluczowe:

PHBV, Fiber, Thin film, Osteoblast, Fibroblast, Cell, attachment

Afiliacje autorów:

Kaniuk Ł.-other affiliation
Krysiak Z.-other affiliation
Sara M.-other affiliation
Stachewicz U.-AGH University of Science and Technology (PL)
140p.
17.Sara M., Sara F., Silvia S., Krysiak Z., Kaniuk Ł., Marzec M., Sung Kyun K., Szewczyk P., Gruszczyński A., Wytrwal-Sarna M., Karbowniczek J., Bernasik A., Sohini K., Stachewicz U., Surface potential and roughness controlled cell adhesion and collagen formation in electrospun PCL fibers for bone regeneration, MATERIALS AND DESIGN, ISSN: 0264-1275, DOI: 10.1016/j.matdes.2020.108915, Vol.194, pp.108915-1-11, 2020

Streszczenie:

Surface potential of biomaterials is a key factor regulating cell responses, driving their adhesion and signaling in tissue regeneration. In this study we compared the surface and zeta potential of smooth and porous electrospun polycaprolactone (PCL) fibers, as well as PCL films, to evaluate their significance in bone regeneration. The ’ surface potential of the fibers was controlled by applying positive and negative voltage polarities during the electrospinning. The surface properties of the different PCL fibers and films were measured using X-ray photoelectron spectroscopy (XPS) and Kelvin probe force microscopy (KPFM), and the zeta potential was measured using the electrokinetic technique. The effect of surface potential on the morphology of bone cells was examined using advanced microcopy, including 3D reconstruction based on a scanning electron microscope with a focused ion beam (FIB-SEM). Initial cell adhesion and collagen formation were studied using fluorescence microscopy and Sirius Red assay respectively, while calcium mineralization was confirmed with energy-dispersive x-ray (EDX) and Alzarin Red staining. These studies revealed that cell adhesion is driven by both the surface potential and morphology of PCL fibers. Furthermore, the ability to tune the surface potential of electrospun PCL scaffolds provides an essential electrostatic handle to enhance cell-material interaction and cellular activity, leading to controllable morphological changes.

Słowa kluczowe:

Surface potential, Kelvin probe force microscopy, Zeta potential, Cells, Adhesion, Mineralization

Afiliacje autorów:

Sara M.-other affiliation
Sara F.-other affiliation
Silvia S.-other affiliation
Krysiak Z.-other affiliation
Kaniuk Ł.-other affiliation
Marzec M.-other affiliation
Sung Kyun K.-other affiliation
Szewczyk P.-other affiliation
Gruszczyński A.-other affiliation
Wytrwal-Sarna M.-other affiliation
Karbowniczek J.-other affiliation
Bernasik A.-other affiliation
Sohini K.-other affiliation
Stachewicz U.-AGH University of Science and Technology (PL)
18.Krysiak Z., Kaniuk Ł., Sara M., Szewczyk P., Sroczyk E., Petra P., Lisiecka-Graca P., Bailey R., Emiliano B., Stachewicz U., Nano- and Microfiber PVB Patches as Natural Oil Carriers for Atopic Skin Treatment, ACS Applied Bio Materials, ISSN: 2576-6422, DOI: 10.1021/acsabm.0c00854, Vol.3, No.11, pp.7666-7676, 2020

Streszczenie:

Atopic dermatitis (eczema) is a widespread disorder, with researchers constantly looking for more efficacious treatments. Natural oils are reported to be an effective therapy for dry skin, and medical textiles can be used as an alternative or supporting therapy. In this study, fibrous membranes from poly(vinyl butyral-co-vinyl alcohol-co-vinyl acetate) (PVB) with low and high molecular weights were manufactured to obtain nano- and micrometer fibers via electrospinning for the designed patches used as oil carriers for atopic skin treatment. The biocompatibility of PVB patches was analyzed using proliferation tests and scanning electron microscopy (SEM), which combined with a focused ion beam (FIB) allowed for the 3D visualization of patches. The oil spreading tests with evening primrose, black cumin seed, and borage were verified with cryo-SEM, which showed the advantage nanofibers have over microfibers as carriers for low-viscosity oils. The skin tests expressed the usability and the enhanced oil delivery performance for electrospun patches. We demonstrate that through the material nano- and microstructure, commercially available polymers such as PVB have great potential to be deployed as a biomaterial in medical applications, such as topical treatments for chronic skin conditions.

Słowa kluczowe:

PVB, electrospun fibers, biocompatibility, oil carriers, atopic skin patches

Afiliacje autorów:

Krysiak Z.-other affiliation
Kaniuk Ł.-other affiliation
Sara M.-other affiliation
Szewczyk P.-other affiliation
Sroczyk E.-other affiliation
Petra P.-other affiliation
Lisiecka-Graca P.-other affiliation
Bailey R.-other affiliation
Emiliano B.-other affiliation
Stachewicz U.-AGH University of Science and Technology (PL)
19.Szewczyk P., Sara M., Krysiak Z., Kaniuk Ł., Karbowniczek J., Stachewicz U., Enhanced osteoblasts adhesion and collagen formation on biomimetic polyvinylidene fluoride (PVDF) films for bone regeneration, Biomedical Materials, ISSN: 1748-6041, DOI: 10.1088/1748-605X/ab3c20, Vol.14, No.6, pp.065006-1-8, 2019

Streszczenie:

Bone tissue engineering can be utilized to study the early events of osteoconduction. Fundamental research in cell adhesion to various geometries and proliferation has shown the potential of extending it to implantable devices for regenerative medicine. Following this concept in our studies, first, we developed well-controlled processing of polyvinylidene fluoride (PVDF) film to obtain a surface biomimicking ECM. We optimized the manufacturing dependent on humidity and temperature during spin-coating of a polymer solution. The mixture of solvents such as dimethylacetamide and acetone together with high humidity conditions led to a biomimetic, highly porous and rough surface, while with lower humidity and high temperatures drying allowed us to obtain a smooth and flat PVDF film. The roughness of the PVDF film was biofabricated and compared to smooth films in cell culture studies for adhesion and proliferation of osteoblasts. The bioinspired roughness of our films enhanced the osteoblast adhesion by over 44%, and there was collagen formation already after 7 days of cell culturing that was proved via scanning electron microscopy observation, light microscopy imaging after Sirius Red staining, and proliferation test such as MTS. Cell development, via extended filopodia, formed profoundly on the rough PVDF surface, demonstrated the potential of the structural design of biomimetic surfaces to enhance further bone tissue regeneration.

Słowa kluczowe:

PVDF, film, roughness, cell, adhesion, collagen

Afiliacje autorów:

Szewczyk P.-other affiliation
Sara M.-other affiliation
Krysiak Z.-other affiliation
Kaniuk Ł.-other affiliation
Karbowniczek J.-other affiliation
Stachewicz U.-AGH University of Science and Technology (PL)
70p.