| Partner: J. E Karbowniczek |
Ostatnie publikacje
| 1. | Sara M.♦, Sara F.♦, Silvia S.♦, Krysiak Z.♦, Kaniuk Ł.♦, Marzec M.♦, Sung Kyun K.♦, Szewczyk P.♦, Gruszczyński A.♦, Wytrwal-Sarna M.♦, Karbowniczek J.♦, Bernasik A.♦, Sohini K.♦, Stachewicz U.♦, Surface potential and roughness controlled cell adhesion and collagen formation in electrospun PCL fibers for bone regeneration, MATERIALS AND DESIGN, ISSN: 0264-1275, DOI: 10.1016/j.matdes.2020.108915, Vol.194, pp.108915-1-11, 2020 Streszczenie: Surface potential of biomaterials is a key factor regulating cell responses, driving their adhesion and signaling in tissue regeneration. In this study we compared the surface and zeta potential of smooth and porous electrospun polycaprolactone (PCL) fibers, as well as PCL films, to evaluate their significance in bone regeneration. The ’ surface potential of the fibers was controlled by applying positive and negative voltage polarities during the electrospinning. The surface properties of the different PCL fibers and films were measured using X-ray photoelectron spectroscopy (XPS) and Kelvin probe force microscopy (KPFM), and the zeta potential was measured using the electrokinetic technique. The effect of surface potential on the morphology of bone cells was examined using advanced microcopy, including 3D reconstruction based on a scanning electron microscope with a focused ion beam (FIB-SEM). Initial cell adhesion and collagen formation were studied using fluorescence microscopy and Sirius Red assay respectively, while calcium mineralization was confirmed with energy-dispersive x-ray (EDX) and Alzarin Red staining. These studies revealed that cell adhesion is driven by both the surface potential and morphology of PCL fibers. Furthermore, the ability to tune the surface potential of electrospun PCL scaffolds provides an essential electrostatic handle to enhance cell-material interaction and cellular activity, leading to controllable morphological changes. Słowa kluczowe: Surface potential, Kelvin probe force microscopy, Zeta potential, Cells, Adhesion, Mineralization Afiliacje autorów:
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| 2. | Szewczyk P.♦, Sara M.♦, Krysiak Z.♦, Kaniuk Ł.♦, Karbowniczek J.♦, Stachewicz U.♦, Enhanced osteoblasts adhesion and collagen formation on biomimetic polyvinylidene fluoride (PVDF) films for bone regeneration, Biomedical Materials, ISSN: 1748-6041, DOI: 10.1088/1748-605X/ab3c20, Vol.14, No.6, pp.065006-1-8, 2019 Streszczenie: Bone tissue engineering can be utilized to study the early events of osteoconduction. Fundamental research in cell adhesion to various geometries and proliferation has shown the potential of extending it to implantable devices for regenerative medicine. Following this concept in our studies, first, we developed well-controlled processing of polyvinylidene fluoride (PVDF) film to obtain a surface biomimicking ECM. We optimized the manufacturing dependent on humidity and temperature during spin-coating of a polymer solution. The mixture of solvents such as dimethylacetamide and acetone together with high humidity conditions led to a biomimetic, highly porous and rough surface, while with lower humidity and high temperatures drying allowed us to obtain a smooth and flat PVDF film. The roughness of the PVDF film was biofabricated and compared to smooth films in cell culture studies for adhesion and proliferation of osteoblasts. The bioinspired roughness of our films enhanced the osteoblast adhesion by over 44%, and there was collagen formation already after 7 days of cell culturing that was proved via scanning electron microscopy observation, light microscopy imaging after Sirius Red staining, and proliferation test such as MTS. Cell development, via extended filopodia, formed profoundly on the rough PVDF surface, demonstrated the potential of the structural design of biomimetic surfaces to enhance further bone tissue regeneration. Słowa kluczowe: PVDF, film, roughness, cell, adhesion, collagen Afiliacje autorów:
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