Partner: Paulina Wigner |
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Ostatnie publikacje
1. | Sliwinska A.♦, Sitarek P.♦, Toma M.♦, Czarny P.♦, Synowiec E.♦, Krupa R.♦, Wigner P.♦, Bialek K.♦, Kwiatkowski D.♦, Korycinska A.♦, Majsterek I.♦, Szemraj J.♦, Galecki P.♦, Sliwinski T.♦, Decreased expression level of BER genes in Alzheimer's disease patients is not derivative of their DNA methylation status, PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY, ISSN: 0278-5846, DOI: 10.1016/j.pnpbp.2017.07.010, Vol.79, pp.311-316, 2017 Streszczenie: Background: Neurodegeneration in Alzheimer's disease can be caused by accumulation of oxidative DNA damage resulting from altered expression of genes involved in the base excision repair system (BER). Promoter methylation can affect the profile of BER genes expression. Decreased expression of BER genes was observed in the brains of AD patients. Słowa kluczowe: Alzheimer's disease, DNA base excision repair genes, Gene expression, Promoter methylation Afiliacje autorów:
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2. | Sliwinska A.♦, Kwiatkowski D.♦, Czarny P.♦, Toma M.♦, Wigner P.♦, Drzewoski J.♦, Fabianowska-Majewska K.♦, Szemraj J.♦, Maes M.♦, Gałecki P.♦, Śliwiński T.♦, The levels of 7,8-dihydrodeoxyguanosine (8-oxoG) and 8-oxoguanine DNA glycosylase 1 (OGG1) - A potential diagnostic biomarkers of Alzheimer's disease, JOURNAL OF THE NEUROLOGICAL SCIENCES, ISSN: 0022-510X, DOI: 10.1016/j.jns.2016.07.008, Vol.368, pp.155-159, 2016 Streszczenie: Evidence indicates that oxidative stress contributes to neuronal cell death in Alzheimer's disease (AD). Increased oxidative DNA damage I, as measured with 8-oxoguanine (8-oxoG), and reduced capacity of proteins responsible for removing of DNA damage, including 8-oxoguanine DNA glycosylase 1 (OGG1), were detected in brains of AD patients. In the present study we assessed peripheral blood biomarkers of oxidative DNA damage, i.e. 8-oxoG and OGG1, in AD diagnosis, by comparing their levels between the patients and the controls. Our study was performed on DNA and serum isolated from peripheral blood taken from 100 AD patients and 110 controls. For 8-oxoG ELISA was employed. The OGG1 level was determined using ELISA and Western blot technique. Levels of 8-oxoG were significantly higher in DNA of AD patients. Both ELISA and Western blot showed decreased levels of OGG1 in serum of AD patients. Our results show that oxidative DNA damage biomarkers detected in peripheral tissue could reflect the changes occurring in the brain of patients with AD. These results also suggest that peripheral blood samples may be useful to measure oxidative stress biomarkers in AD. Słowa kluczowe: Alzheimer's disease, Oxidative stress, Oxidative DNA damage, 7 8-dihydrodeoxyguanosine (8-oxoG), DNA base excision repair, 8-oxoguanine DNA glycosylase 1 (OGG1) Afiliacje autorów:
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