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Ostatnie publikacje
1. | Kwiatkowski D.♦, Czarny P.♦, Toma M.♦, Jurkowska N.♦, Śliwinska A.♦, Drzewoski J.♦, Bachurska A.♦, Szemraj J.♦, Maes M.♦, Berk M.♦, Su K.P.♦, Gałecki P.♦, Śliwiński T.♦, Associations between DNA Damage, DNA Base Excision Repair Gene Variability and Alzheimer's Disease Risk, DEMENTIA AND GERIATRIC COGNITIVE DISORDERS, ISSN: 1420-8008, DOI: 10.1159/000443953, Vol.41, No.3-4, pp.152-171, 2016 Streszczenie: Background: Increased oxidative damage to DNA is one of the pathways involved in Alzheimer's disease (AD). Insufficient base excision repair (BER) is in part responsible for increased oxidative DNA damage. The aim of the present study was to assess the effect of polymorphic variants of BER-involved genes and the peripheral markers of DNA damage and repair in patients with AD. Material and Methods: Comet assays and TaqMan probes were used to assess DNA damage, BER efficiency and polymorphic variants of 12 BER genes in blood samples from 105 AD patients and 130 controls. The DNA repair efficacy (DRE) was calculated according to a specific equation. Results: The levels of endogenous and oxidative DNA damages were higher in AD patients than controls. The polymorphic variants of XRCC1 c.580C>T XRCC1 c.1196A>G and OGG1 c.977C>G are associated with increased DNA damage in AD. Conclusion: Our results show that oxidative stress and disturbances in DRE are particularly responsible for the elevated DNA lesions in AD. The results suggest that oxidative stress and disruption in DNA repair may contribute to increased DNA damage in AD patients and risk of this disease. In addition, disturbances in DRE may be associated with polymorphisms of OGG1 and XRCC1. Słowa kluczowe: DNA damage, DNA base excision repair, Alzheimer's disease risk, Dementia, Oxidative stress Afiliacje autorów:
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2. | Czarny P.♦, Kwiatkowski D.♦, Gałecki P.♦, Talarowska M.♦, Orzechowska A.♦, Bobińska K.♦, Bielecka-Kowalska A.♦, Szemraj J.♦, Maes M.♦, Su K.P.♦, Śliwiński T.♦, Association between single nucleotide polymorphisms of MUTYH, hOGG1 and NEIL1 genes, and depression, JOURNAL OF AFFECTIVE DISORDERS, ISSN: 0165-0327, DOI: 10.1016/j.jad.2015.05.044, Vol.184, pp.90-96, 2015 Streszczenie: Background: An elevated levels oxidative modified DNA bases and a decreased efficiency of oxidative DNA damage repair were found in patients with depression disorders, including recurrent type (rDD). The glycosylases are involved in base excision repair (BER), which eliminates oxidative DNA damage. Therefore, we genotyped the single nucleotide polymorphisms (SNPs) of genes encoding three glycosylases: hOGG1, MUTYH and NEILL Słowa kluczowe: Depression, Glycosylases, BER, DNA repair, DNA damage Afiliacje autorów:
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