Partner: W. Białobrzeska |
Ostatnie publikacje
1. | Białobrzeska W.♦, Firganek D.♦, Czerkies M., Lipniacki T., Skwarecka M.♦, Dziąbowska K.♦, Cebula Z.♦, Malinowska N.♦, Bigus D.♦, Bięga E.♦, Pyrć K.♦, Pala K.♦, Żołędowska S.♦, Nidzworski D.♦, Electrochemical immunosensors based on screen-printed gold and glassy carbon electrodes: comparison of performance for respiratory syncytial virus detection, Biosensors, ISSN: 2079-6374, DOI: 10.3390/bios10110175, Vol.10, No.11, pp.175-1-13, 2020 Streszczenie: This paper presents the development and comparison of label-free electrochemical immunosensors based on screen-printed gold and glassy carbon (GC) disc electrodes for efficient and rapid detection of respiratory syncytial virus (RSV). Briefly, the antibody specific to the F protein of RSV was successfully immobilized on modified electrodes. Antibody coupling on the Au surface was conducted via 4-aminothiophenol (4-ATP) and glutaraldehyde (GA). The GC surface was modified with poly-L-lysine (PLL) for direct anti-RSV conjugation after EDC/NHS (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-Hydroxysuccinimide) activation. Electrochemical characterizations of the immunosensors were carried out by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). GC-based immunosensors show a dynamic range of antigen detection from 1.0 × 10^5 PFU/mL to 1.5×10^7 PFU/mL, more than 1.0 × 10^5 PFU/mL to 1.0 × 10^7 PFU/mL for the Au-based sensor. However, the GC platform is less sensitive and shows a higher detection limit (LOD) for RSV. The limit of detection of the Au immunosensor is 1.1 × 10^3 PFU/mL, three orders of magnitude lower than 2.85 × 10^6 PFU/mL for GC. Thus, the Au-based immunosensor has better analytical performance for virus detection than a carbon-based platform due to high sensitivity and very low RSV detection, obtained with good reproducibility. Słowa kluczowe: respiratory syncytial virus, cyclic voltammetry, electrochemical impedance spectroscopy, sensor, gold electrode, glassy carbon Afiliacje autorów:
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