Instytut Podstawowych Problemów Techniki

Streszczenie:

Nibrin plays an important role in the DNA damage response (DDR) and DNA repair. DDR is a crucial signaling pathway in apoptosis and senescence. To verify whether truncated nibrin (p70), causing Nijmegen Breakage Syndrome (NBS), is involved in DDR and cell fate upon DNA damage, we used two (S4 and S3R) spontaneously immortalized T cell lines from NBS patients, with the founding mutation and a control cell line (L5). S4 and S3R cells have the same level of p70 nibrin, however p70 from S4 cells was able to form more complexes with ATM and BRCA1. Doxorubicin-induced DDR followed by cell senescence could only be observed in L5 and S4 cells, but not in the S3R ones. Furthermore the S3R cells only underwent cell death, but not senescence after doxorubicin treatment. In contrary to doxorubicin treatment, cells from all three cell lines were able to activate the DDR pathway after being exposed to γ-radiation. Downregulation of nibrin in normal human vascular smooth muscle cells (VSMCs) did not prevent the activation of DDR and induction of senescence. Our results indicate that a substantially reduced level of nibrin or its truncated p70 form is sufficient to induce DNA-damage dependent senescence in VSMCs and S4 cells, respectively. In doxorubicin-treated S3R cells DDR activation was severely impaired, thus preventing the induction of senescence.

Afiliacje autorów:

Streszczenie:

Senescence of vascular smooth muscle cells (VSMCs) contributes to aging as well as age-related diseases of the cardiovascular system. Senescent VSMCs have been shown to be present in atherosclerotic plaques. Both replicative (RS) and stress-induced premature senescence (SIPS) accompany cardiovascular diseases. We aimed to establish the signature of RS and SIPS of VSMCs, induced by a common anticancer drug, doxorubicin, and to discover the so far undisclosed features of senescent cells that are potentially harmful to the organism. Most of the senescence hallmarks were common for both RS and SIPS; however, some differences were observed. 32 % of doxorubicin-treated cells were arrested in the G2/M phase of the cell cycle, while 73 % of replicatively senescing cells were arrested in the G1 phase. Moreover, on the basis of alkaline phosphatase activity measurements, we show that a 7-day treatment with doxorubicin (dox), does not cause precocious cell calcification, which is a characteristic feature of RS. We did not observe calcification even though after 7 days of dox-treatment many other markers characteristic for senescent cells were present. It can suggest that dox-induced SIPS does not accelerate the mineralization of vessels. We consider that detailed characterization of the two types of cellular senescence can be useful in in vitro studies of potential anti-aging factors.

Słowa kluczowe:

Senescence, VSMCs, Doxorubicin, Aging, Cardiovascular diseases, Calcification

Afiliacje autorów:

Streszczenie:

Materiał genetyczny każdego organizmu jest ciągle narażony na uszkodzenia spowodowane fizjologicznymi procesami zachodzącymi w komórce, jaki i działaniem szkodliwych czynników pochodzących z otaczającego środowiska. W komórkach eukariotycznych został rozwinięty system monitorujący integralność genomu, nazwany szlakiem odpowiedzi na uszkodzenia DNA (DDR). Odpowiedź komórek na działanie czynnika indukującego uszkodzenia DNA może prowadzić do starzenia, naprawy uszkodzeń DNA, bądź śmierci komórkowej. Kluczowym elementem szlaku DDR, jest białko p53. W wyniku licznych modyfikacji potranslacyjnych zaangażowane może ono być w aktywację wielu genów i białek, prowadzących do przeżycia lub śmierci komórki. W starzeniu komórkowym białko p53 prowadzi do indukcji p21, czego konsekwencją jest zatrzymanie komórek w cyklu. W apoptozie białko p53 bierze udział w aktywacji kaspaz, które odpowiedzialne są za degradację wielu białek. Decyzja o tym, która ze ścieżek będzie aktywowana zależy od stopnia uszkodzenia DNA.

Słowa kluczowe:

starzenie komórkowe, apoptoza, szlak odpowiedzi na uszkodzenia DNA, autofagia, naprawa uszkodzeń DNA

Afiliacje autorów:

Streszczenie:

Starzenie jest złożonym procesem związanym z nieodwracalnym zatrzymaniem komórek w cyklu. Wyróżnić można starzenie replikacyjne, zależne od skracania telomerów, i starzenie przyspieszone, które jest niezależne od ich skracania. Starzenie replikacyjne obserwowane jest w hodowli jako skończona liczba podziałów komórek, co może trwać od kilku tygodni do miesięcy, w zależności od typu komórek. Starzenie przyspieszone zachodzi już po kilku dniach od zadziałania czynnika je indukującego. Nie ma jednego uniwersalnego znacznika procesu starzenia komórkowego. Badanie starzenia komórkowego możliwe jest dzięki występowaniu szeregu znaczników tego procesu, które pozwalają obserwować zmiany zarówno na poziomie molekularnym jak i biochemicznym. Dopiero ich współwystępowanie pozwala na uzyskanie pewności, że komórki uległy starzeniu.

Słowa kluczowe:

znaczniki starzenia, starzenie replikacyjne, starzenie przyspieszone, fenotyp sekrecyjny, β-galaktozydaza związana ze starzeniem

Afiliacje autorów:

Streszczenie:

Curcumin, a phytochemical derived from the rhizome of Curcuma longa, is a very potent inducer of cancer cell death. It is believed that cancer cells are more sensitive to curcumin treatment than normal cells. Curcumin has been shown to act as a prooxidant and induce DNA lesions in normal cells. We were interested in whether curcumin induces DNA damage and the DNA damage response (DDR) signalling pathway leading to apoptosis in normal resting human T cells. To this end, we analysed DNA damage after curcumin treatment of resting human T cells (CD3+) and of proliferating leukaemic Jurkat cells by the fluorimetric detection of alkaline DNA unwinding (FADU) assay and immunocytochemical detection of γ-H2AX foci. We showed that curcumin-treated Jurkat cells and resting T cells showed neither DNA lesions nor did they activate key proteins in the DDR signalling pathway, such as phospho-ATM and phospho-p53. However, both types of cell were equally sensitive to curcumin-induced apoptosis and displayed activation of caspase-8 but not of DNA damage-dependent caspase-2. Altogether, our results revealed that curcumin can induce apoptosis of normal resting human T cells that is not connected with DNA damage.

Afiliacje autorów:

Streszczenie:

It is believed that normal cells with an unaffected DNA damage response (DDR) and DNA damage repair machinery, could be less prone to DNA damaging treatment than cancer cells. However, the anticancer drug, etoposide, which is a topoisomerase II inhibitor, can generate DNA double strand breaks affecting not only replication but also transcription and therefore can induce DNA damage in non-replicating cells. Indeed, we showed that etoposide could influence transcription and was able to activate DDR in resting human T cells by inducing phosphorylation of ATM and its substrates, H2AX and p53. This led to activation of PUMA, caspases and to apoptotic cell death. Lymphoblastoid leukemic Jurkat cells, as cycling cells, were more sensitive to etoposide considering the level of DNA damage, DDR and apoptosis. Next, we used ATM inhibitor, KU 55933, which has been shown previously to be a radio/chemo-sensitizing agent. Pretreatment of resting T cells with KU 55933 blocked phosphorylation of ATM, H2AX and p53, which, in turn, prevented PUMA expression, caspase activation and apoptosis. On the other hand, KU 55933 incremented apoptosis of Jurkat cells. However, etoposide-induced DNA damage in resting T cells was not influenced by KU 55933 as revealed by the FADU assay. Altogether our results show that KU 55933 blocks DDR and apoptosis induced by etoposide in normal resting T cells, but increased cytotoxic effect on proliferating leukemic Jurkat cells. We discuss the possible beneficial and adverse effects of drugs affecting the DDR in cancer cells that are currently in preclinical anticancer trials.

Słowa kluczowe:

FADU, γH2AX, DSBs, Caspases, KU 55933

Afiliacje autorów: