Instytut Podstawowych Problemów Techniki

Słowa kluczowe:

antioxidant capacity, Chrysanthemum × morifolium /Ramat./ Hemsl., molecular markers, nanotechnology, polyphenols, SCoT

Afiliacje autorów:

Streszczenie:

Purpose: Chrysanthemum is one of the most popular ornamental plants worldwide. Its breeding remains a highly relevant topic. Nanotechnology significantly and interdisciplinarily contributes to the progress in modern horticulture. To date, there are no studies on the use of the proposed heavy metal-based nanoparticles in mutation breeding of ornamental plants. Methods: CdS NPs, Co3 O4 NPs, and Fe 3O 4@Co NPs were synthesized and applied at a concentration of 75 mg·L −1 in the in vitro internode culture of Chrysanthemum × morifolium (Ramat). Hemsl. ‘Lilac Wonder’. Results: The highest number of adventitious shoots was regenerated on the control and Fe3 O 4@Co NP-treated internodes, whereas the use of CdS NPs and Co3 O 4 NPs hampered regeneration. The NP-treated shoots, compared to the control, accumulated less flavonols and more anthocyanins and polyphenols, and exhibited increased antioxidant capacity. The highest activity of oxidative stress enzymes and the lowest chlorophyll content were noted in CdS NP-treated shoots. The tested nanoparticles also affected the further growth and development of plants during ex vitro greenhouse cultivation. The longest stems were found in Fe 3 O4 @Co NP-treated plants, contrary to CdS NPs and Co3 O4 NPs. The CdS NP-treated plants developed leaves with the smallest surface area, perimeter, length, and width. Evaluation of inflorescences revealed quantitative changes in anthocyanins content. The highest pigment content was found in ligulate flowers of Fe 3 O4 @Co NP-treated plants. One individual with variegated leaves was phenotypically identified within Co3 O 4 NP-treated plants. Genetic variation was detected in 7–8.1% of the plants studied. The SCoT marker system generated more bands and polymorphisms than RAPD. PCoA analysis revealed distinct genetic groupings, with the most altered genotype (treated with CdS NPs) classified as polymorphic by both marker systems. The other 11 polymorphic genotypes did not overlap between RAPD and SCoT analyses. Conclusion: Our results proved that nanoparticles can serve as a novel and valuable tool for plant breeding.

Słowa kluczowe:

breeding, Chrysanthemum × morifolium, genetic marker, nanobiotechnology, phenotype

Afiliacje autorów:

Słowa kluczowe:

Axillary buds, Nanoparticle–plant interaction, Plant biotechnology, RAPD, Secondary metabolism regulation, Synthetic seeds

Afiliacje autorów:

Streszczenie:

Chrysanthemum × morifolium /Ramat./ Hemsl. is one of the most popular ornamental plants in the world, and its breeding remains a highly relevant topic. Recent years have been marked by the intensive development of nanotech-nology. Nanoparticles (NPs) easily interact with cells of living organisms, causing various effects. The research aim was to answer the question of whether nanoparticles can be used in chrysanthemum breeding as factors inducing vari-ability. In the experiment, CdS NPs, Co3O4 NPs, and Fe3O4@Co NPs were applied at the concentration of 75 mg·L-1
in the in vitro culture of internodes of chrysanthemum ‘Lilac Wonder’. The regeneration efficiency was determined,and the biochemical profile and stress response of adventitious shoots were verified. During further ex vitro cultiva-tion in the greenhouse, the color and type of inflorescence were assessed, and biometric measurements of the plants were performed. Genetic variability was verified using RADP and SCoT molecular markers. The use of CdS NPs and Co3O4 NPs limited the regeneration efficiency. The NPs-treated shoots accumulated less flavonols, more anthocyanins and polyphenols, and showed increased antioxidant capacity. The highest activity of oxidative stress enzymes (APOX,GPOX, SOD), and the lowest chlorophyll content were noted in CdS NP-treated shoots. The tested NPs also affected the further growth of plants during ex vitro cultivation. The longest stems were found in plants treated with Fe3O4@Co NPs, contrary to CdS NPs and Co3O4 NPs. The CdS NP-treated plants developed leaves with the smallest surface area,
perimeter, length, and width. Evaluation of inflorescences did not reveal qualitative variations in the color of ligulate
florets, however, quantitative changes related to anthocyanins content were noticed. The highest pigment content was found in ligulate flowers of Fe3O4@Co NP-treated plants. One individual (mutant) was phenotypically identified within Co3O4 NP-treated plants, with variegated leaves. The use of nanoparticles also induced genetic variation, with CdS NPs causing the most distinct polymorphic changes, as confirmed by both RAPD and SCoT markers. While Fe3O4@Co NPs and Co3O4 NPs also generated polymorphic genotypes, their effects were less evident, suggesting that NPs vary in mutagenic potential, but could serve as a novel tool for plant breeding.

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